对UmuD共价二聚体中RecA介导的切割的抑制作用。
Inhibition of RecA-mediated cleavage in covalent dimers of UmuD.
作者信息
Lee M H, Guzzo A, Walker G C
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
出版信息
J Bacteriol. 1996 Dec;178(24):7304-7. doi: 10.1128/jb.178.24.7304-7307.1996.
Abstract
Disulfide-cross-linked UmuD2 derivatives were cleaved poorly upon incubation with activated RecA. Reducing the disulfide bonds prior to incubating the derivatives with RecA dramatically increased their extent of cleavage. These observations suggest that the UmuD monomer is a better substrate for the RecA-mediated cleavage reaction than the dimer.
摘要
与活化的RecA一起温育时，二硫键交联的UmuD2衍生物裂解效果不佳。在将衍生物与RecA温育之前还原二硫键，可显著提高其裂解程度。这些观察结果表明，与二聚体相比，UmuD单体是RecA介导的裂解反应更好的底物。
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