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J Bacteriol. 1996 Dec;178(24):7308-10. doi: 10.1128/jb.178.24.7308-7310.1996.
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本文引用的文献

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Vitamin B(12) in photosynthetic bacteria and methionine synthesis by Rhodopseudomonas spheroides.光合细菌中的维生素 B(12)和球形红假单胞菌的蛋氨酸合成。
Biochem J. 1967 Mar;102(3):774-81. doi: 10.1042/bj1020774.
2
Heterologous expression of the bchM gene product from Rhodobacter capsulatus and demonstration that it encodes S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase.来自荚膜红细菌的bchM基因产物的异源表达及其编码S-腺苷-L-甲硫氨酸:镁原卟啉IX甲基转移酶的证明。
J Bacteriol. 1994 Sep;176(17):5290-6. doi: 10.1128/jb.176.17.5290-5296.1994.
3
nif gene expression studies in Rhodobacter capsulatus: ntrC-independent repression by high ammonium concentrations.荚膜红细菌中固氮酶基因表达研究:高铵浓度下不依赖ntrC的抑制作用
Mol Microbiol. 1993 Oct;10(1):123-32. doi: 10.1111/j.1365-2958.1993.tb00909.x.
4
Identification and sequence analysis of genes involved in late steps in cobalamin (vitamin B12) synthesis in Rhodobacter capsulatus.荚膜红细菌中参与钴胺素(维生素B12)合成后期步骤的基因的鉴定与序列分析。
J Bacteriol. 1995 Aug;177(15):4481-7. doi: 10.1128/jb.177.15.4481-4487.1995.
5
Biosynthesis of carotenoids derived from neurosporene in Rhodopseudomonas capsulata.荚膜红假单胞菌中源自八氢番茄红素的类胡萝卜素生物合成。
J Biol Chem. 1980 Mar 25;255(6):2427-32.
6
Magnesium protoporphyrin chelatase activity in Rhodopseudomonas spheroides. Studies with whole cells.球形红假单胞菌中镁原卟啉螯合酶活性。全细胞研究。
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7
Carotenoid biosynthesis in Rhodopseudomonas spheroides. S-adenosylmethionine as the methylating agent in the biosynthesis of spheroidene and spheroidenone.球形红假单胞菌中的类胡萝卜素生物合成。S-腺苷甲硫氨酸作为球形烯和球形烯酮生物合成中的甲基化剂。
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8
Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria.土壤和水生细菌的插入诱变:用于革兰氏阴性菌体外插入诱变的一族DNA片段。
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荚膜红细菌的bluF基因参与了钴胺酰胺向钴胺素(维生素B12)的转化过程。

The bluF gene of Rhodobacter capsulatus is involved in conversion of cobinamide to cobalamin (vitamin B12).

作者信息

Pollich M, Wersig C, Klug G

机构信息

Institut für Mikro- und Molekularbiologie, Giessen, Germany.

出版信息

J Bacteriol. 1996 Dec;178(24):7308-10. doi: 10.1128/jb.178.24.7308-7310.1996.

DOI:10.1128/jb.178.24.7308-7310.1996
PMID:8955417
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC178648/
Abstract

The bluF gene of Rhodobacter capsulatus is the first gene of the bluFEDCB operon which is involved in late steps of the cobalamin synthesis. To determine the function of the bluF gene product, a bluF::omega-Km mutant strain was constructed and characterized. This vitamin B12 auxotrophic mutant strain shows a 3.5-times higher vitamin B12 requirement under phototrophic growth conditions than under chemotrophic growth conditions. Surprisingly, the bluF promoter activity does not respond to alterations to the oxygen tension or vitamin B12 concentration.

摘要

荚膜红细菌的bluF基因是bluFEDCB操纵子的第一个基因,该操纵子参与钴胺素合成的后期步骤。为了确定bluF基因产物的功能,构建并表征了一个bluF::omega-Km突变菌株。这个维生素B12营养缺陷型突变菌株在光合生长条件下对维生素B12的需求比在化能营养生长条件下高3.5倍。令人惊讶的是,bluF启动子活性对氧张力或维生素B12浓度的变化没有反应。