荚膜红细菌的bluF基因参与了钴胺酰胺向钴胺素（维生素B12）的转化过程。
The bluF gene of Rhodobacter capsulatus is involved in conversion of cobinamide to cobalamin (vitamin B12).
作者信息
Pollich M, Wersig C, Klug G
机构信息
Institut für Mikro- und Molekularbiologie, Giessen, Germany.
出版信息
J Bacteriol. 1996 Dec;178(24):7308-10. doi: 10.1128/jb.178.24.7308-7310.1996.
Abstract
The bluF gene of Rhodobacter capsulatus is the first gene of the bluFEDCB operon which is involved in late steps of the cobalamin synthesis. To determine the function of the bluF gene product, a bluF::omega-Km mutant strain was constructed and characterized. This vitamin B12 auxotrophic mutant strain shows a 3.5-times higher vitamin B12 requirement under phototrophic growth conditions than under chemotrophic growth conditions. Surprisingly, the bluF promoter activity does not respond to alterations to the oxygen tension or vitamin B12 concentration.
摘要
荚膜红细菌的bluF基因是bluFEDCB操纵子的第一个基因，该操纵子参与钴胺素合成的后期步骤。为了确定bluF基因产物的功能，构建并表征了一个bluF::omega-Km突变菌株。这个维生素B12营养缺陷型突变菌株在光合生长条件下对维生素B12的需求比在化能营养生长条件下高3.5倍。令人惊讶的是，bluF启动子活性对氧张力或维生素B12浓度的变化没有反应。
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