Aravina L A, Ponomareva V D
Mikrobiologiia. 1977 May-Jun;46(3):472-7.
The effect of inoculation material on biosynthesis of proteolytic and amylolytic enzymes was studied with Aspergillus terricola H-20. The highest yield of proteolytic enzymes was found with the inoculation material obtained on oatmeal flakes with single shaking of the substrate. The highest number of viable conidia was detected by the sixth day of the growth; they favoured the synthesis of both proteases and amylases. The maximum yield of proteases was found when the density of inoculation was 6-10(6) conidia per 1 ml of the medium. The proteolytic activity of the fungus decreased after storage in a physiological solution at +4 degrees C for over 5 days; the amylolytic activity decreased if the conidia were kept under these conditions for two days.
研究了接种材料对土曲霉H-20生物合成蛋白水解酶和淀粉水解酶的影响。在燕麦片上获得的接种材料,经底物单次振荡后,蛋白水解酶产量最高。生长到第6天检测到的活分生孢子数量最多;它们有利于蛋白酶和淀粉酶的合成。当接种密度为每1毫升培养基6-10(6)个分生孢子时,蛋白酶产量最高。真菌在4℃生理溶液中储存超过5天后,其蛋白水解活性下降;如果分生孢子在这些条件下保存两天,淀粉水解活性下降。