Hallén L, Johansson C, Laurent C, Dahlqvist A
Department of Otorhinolaryngology, Central Hospital, Falun, Sweden.
Anat Rec. 1996 Dec;246(4):441-5. doi: 10.1002/(SICI)1097-0185(199612)246:4<441::AID-AR3>3.0.CO;2-Y.
The larynx is a complex organ composed of different connective tissue elements. So far, the extracellular matrix of the larynx has not been thoroughly described. Hyaluronan is a matrix polysaccharide with physicochemical effects and biological cell functions in soft connective tissues.
The histochemical distribution of hyaluronan (hyaluronic acid, hyaluronate) was studied in tissue sections from various levels of the rabbit larynx by means of a hyaluronan-binding protein and avidin biotin peroxidase staining. Microwave-aided fixation was used to retain the extracellular location of hyaluronan.
Hyaluronan accumulated chiefly in the subepithelial lamina propria and in the connective tissue enclosing striated muscle fibres of the thyroarytenoid muscle and vocalis muscle. This localization contrasted sharply with the weak staining for hyaluronan in muscles external to the thyroid cartilage. Intensive staining for hyaluronan was found in perivascular and periglandular connective tissue, as in the vacuoles of the hyaline cartilage of the thyroid, cricoid and arytenoid cartilages, and to a lesser extent in the lacunae of the chondrocytes and in the perichondrium of the elastic cartilage of the epiglottis.
Hyaluronan was heterogenously distributed in the rabbit larynx. It was abundant in intrinsic laryngeal muscles performing small, precise, and rapid movements and in the subepithelium at the glottic level, where it may facilitate mucosal movements. The abundant hyaluronan in the subglottic region may be involved in the control of vascular leakage and edema formation.
喉是一个由不同结缔组织成分组成的复杂器官。迄今为止，喉的细胞外基质尚未得到充分描述。透明质酸是一种基质多糖，在软结缔组织中具有物理化学作用和生物细胞功能。
采用透明质酸结合蛋白和抗生物素蛋白 - 生物素过氧化物酶染色法，研究透明质酸（透明质酸、透明质酸盐）在兔喉不同水平组织切片中的组织化学分布。使用微波辅助固定以保留透明质酸的细胞外位置。
透明质酸主要积聚在声门下固有层以及包绕甲杓肌和声带肌横纹肌纤维的结缔组织中。这种定位与甲状软骨外肌肉中透明质酸的弱阳性染色形成鲜明对比。在血管周围和腺周结缔组织中发现透明质酸的强染色，如在甲状腺、环状软骨和杓状软骨透明软骨的空泡中，在较小程度上也见于软骨细胞的腔隙和会厌弹性软骨的软骨膜中。
透明质酸在兔喉中呈异质性分布。它在执行小幅度、精确且快速运动的喉内肌以及声门水平的上皮下丰富，在那里它可能促进黏膜运动。声门下区域丰富的透明质酸可能参与血管渗漏和水肿形成的控制。
