Schmitt E, Guillon J M, Meinnel T, Mechulam Y, Dardel F, Blanquet S
Laboratoire de Biochimie, URA-CNRS no 1970, Ecole Polytechnique, Palaiseau, France.
Biochimie. 1996;78(7):543-54. doi: 10.1016/s0300-9084(96)80001-0.
Selection of the proper start codon for the synthesis of a polypeptide by the Escherichia coli translation initiation apparatus involves several macromolecular components. These macromolecules interact in a specific and concerted manner to yield the translation initiation complex. This review focuses on recent data concerning the properties of the initiator tRNA and of enzymes and factors involved in the translation initiation process. The three initiation factors, as well as methionyl-tRNA synthetase and methionyl-tRNA(f)Met formyltransferase are described. In addition, the tRNA recognition properties of EF-Tu and peptidyl-tRNA hydrolase are considered. Finally, peptide deformylase and methionine aminopeptidase, which catalyze the amino terminal maturation of nascent polypeptides, can also be associated to the translation initiation process.
大肠杆菌翻译起始装置选择合适的起始密码子来合成多肽涉及多个大分子组分。这些大分子以特定且协同的方式相互作用，以产生翻译起始复合物。本综述聚焦于有关起始tRNA以及翻译起始过程中涉及的酶和因子特性的最新数据。文中描述了三种起始因子，以及甲硫氨酰 - tRNA合成酶和甲硫氨酰 - tRNA(f)Met甲酰基转移酶。此外，还考虑了EF - Tu和肽基 - tRNA水解酶的tRNA识别特性。最后，催化新生多肽氨基末端成熟的肽脱甲酰基酶和甲硫氨酸氨肽酶也可能与翻译起始过程相关。
