Urbain B, Prouvost J F, Beerens D, Michel O, Nicks B, Ansay M, Gustin P
Laboratoire de pharmacologie et d'écotoxicologie vétérinaires, faculté de médecine vétérinaire, université de Liege, Belgium.
Vet Res. 1996;27(6):569-78.
A new experimental setup was developed to expose pigs to dust and airborne endotoxins in an environmental chamber, at levels liable to be encountered in pig farm buildings. The following parameters were evaluated in a chamber containing two pigs of 10 kg body-weight: inhalable and respirable dust gravimetric concentrations were measured using area samplers and expressed as mg/m3. The respirable dust concentration was also measured using a "TM digital microP respirable dust-measuring instrument', which has been shown to give similar results to the gravimetric method. The endotoxin concentration was evaluated using the Limulus-assay and expressed as ng/m3 of air containing the inhalable or respirable dust or as ng/mg of inhalable and respirable dust. Feed flour dust was introduced into the chamber to obtain different concentrations of inhalable and respirable dust ranging from 3.62 to 76.66 mg/m3 and from 0.24 to 1.40 mg/m3, respectively. The endotoxin concentration was modulated by mixing the feed flour with Escherichia coli endotoxins before blowing it into the chamber. The endotoxin concentrations in the air containing inhalable or respirable dust ranged from 28.9 to 270.0 ng/m3 and from 2.22 to 36.38 ng/m3, respectively, depending on the amount of endotoxins added to the dust. Data were also obtained in a piggery. The experimental setup detailed in this paper could be used to study the significance of air contaminants in the development of pig respiratory diseases.
开发了一种新的实验装置，用于在环境舱中将猪暴露于灰尘和空气中的内毒素中，其水平与猪场建筑中可能遇到的水平相当。在一个装有两头体重为10千克猪的舱室内评估了以下参数：使用面积采样器测量可吸入和可呼吸灰尘的重量浓度，并以毫克/立方米表示。还使用“TM数字微型P可呼吸灰尘测量仪”测量可呼吸灰尘浓度，该仪器已被证明与重量法给出的结果相似。使用鲎试剂法评估内毒素浓度，并以含可吸入或可呼吸灰尘的空气中的纳克/立方米表示，或以可吸入和可呼吸灰尘的纳克/毫克表示。将饲料粉尘引入舱室，以分别获得范围为3.62至76.66毫克/立方米和0.24至1.40毫克/立方米的不同浓度的可吸入和可呼吸灰尘。通过在将饲料粉尘吹入舱室之前将其与大肠杆菌内毒素混合来调节内毒素浓度。含可吸入或可呼吸灰尘的空气中的内毒素浓度分别为28.9至270.0纳克/立方米和2.22至36.38纳克/立方米，具体取决于添加到灰尘中的内毒素量。还在一个养猪场中获取了数据。本文详细介绍的实验装置可用于研究空气污染物在猪呼吸道疾病发展中的重要性。
