Georgiou C D
Department of Biology, University of Patras, Greece.
Biotech Histochem. 1996 Nov;71(6):295-7. doi: 10.3109/10520299609117178.
This work describes a new apparatus for growing fungi and other microorganisms on liquid nutrient media in a Petri dish. The apparatus is composed of a net supporting a cellophane membrane stretched between an outer and an inner ring that is placed inside a Petri dish. This modification of the standard Petri dish offers many advantages for studying growth, metabolism, differentiation, and other aspects of fungi in liquid cultures with minimal waste of expensive chemicals. Monitoring of excreted or absorbed substances by the fungi, the aseptic transfer of undisturbed fungal colonies from dish to dish, and harvesting are made easier, using this apparatus.
这项工作描述了一种用于在培养皿中的液体营养培养基上培养真菌和其他微生物的新装置。该装置由一个网组成，该网支撑着一张拉伸在置于培养皿内的外环和内环之间的玻璃纸膜。这种对标准培养皿的改进为研究液体培养中真菌的生长、代谢、分化及其他方面提供了许多优势，同时能将昂贵化学药品的浪费降至最低。使用该装置可以更轻松地监测真菌分泌或吸收的物质、将未受干扰的真菌菌落无菌地从一个培养皿转移到另一个培养皿以及进行收获操作。
