Karlsson A-S, Hedrén M, Almqvist C, Larsson K, Renström A
Department of Occupational Health, National Institute for Working Life, Stockholm, Sweden.
Allergy. 2002 Feb;57(2):164-8. doi: 10.1034/j.1398-9995.2002.1s3297.x.
Dust reservoir sampling is the most commonly used method for assessment of indirect allergen exposure. Because assessment of personal exposure using person-carried pumps is time-consuming and expensive we evaluated the Petri dish sampling method for measurement of airborne cat allergen in classrooms.
Petri dish sampling was evaluated in three study parts. Part I: by comparison between Petri dish sampling and personal air sampling in 44 classrooms with many (> or = 20%) and few (< or = 10%) cat owners and by additional Petri dish sampling in 40 pet-free homes. Part II: by sampling with duplicate Petri dishes in 28 classrooms. Part III: by sampling in three classrooms at four sampling heights during different sampling times. All samples were analyzed for cat allergen (Fel d 1) content with a monoclonal antibody two-site ELISA (enzyme linked immunosorbent assay), using signal amplification when necessary.
There was a significant correlation between Petri dish sampling and personal air sampling (r = 0.66; P < 0.0001). Levels were five-fold higher in classes with many cat owners than in classes with few cat owners, regardless of method. A corresponding difference was found in the homes. Duplicate sample values were in fair agreement (Bland-Altman test) and were correlated (r = 0.77; P < 0.0001). Cumulative levels collected weekly in one Petri dish were lower than using five daily Petri dishes, regardless of sampling height.
Petri dish sampling can be useful as an alternative method to personal air sampling of airborne allergens.
储尘采样是评估间接过敏原暴露最常用的方法。由于使用个人携带式泵评估个人暴露既耗时又昂贵，因此我们评估了培养皿采样法用于测量教室空气中猫过敏原的情况。
培养皿采样在三个研究部分中进行评估。第一部分：通过在44间养猫主人多（≥20%）和养猫主人少（≤10%）的教室中比较培养皿采样和个人空气采样，并在40个无宠物家庭中额外进行培养皿采样。第二部分：在28间教室中使用重复的培养皿进行采样。第三部分：在不同采样时间在三间教室的四个采样高度进行采样。所有样本均使用单克隆抗体双位点ELISA（酶联免疫吸附测定）分析猫过敏原（猫毛屑蛋白1）含量，必要时使用信号放大技术。
培养皿采样和个人空气采样之间存在显著相关性（r = 0.66；P < 0.0001）。无论采用何种方法，养猫主人多的班级中的水平比养猫主人少的班级高五倍。在家庭中也发现了相应的差异。重复样本值一致性良好（布兰德 - 奥特曼检验）且具有相关性（r = 0.77；P < 0.0001）。无论采样高度如何，每周在一个培养皿中收集的累积水平低于使用五个每日培养皿的情况。
培养皿采样可作为空气中过敏原个人空气采样的替代方法。
