García I, Pascual A, Ballesta S, Perea E J
Department of Microbiology, School of Medicine, University of Seville, Spain.
Chemotherapy. 1996 Nov-Dec;42(6):465-7. doi: 10.1159/000239481.
The effect of phagocytosis of Escherichia coli, Staphylococcus aureus and Haemophilus influenzae on the intracellular penetration of sparfloxacin into human polymorphonuclear neutrophils (PMN) was evaluated. The intracellular concentration of sparfloxacin was similar in PMN containing intracellular bacteria and in resting PMN [cellular to extracellular ratio (C/ E) > 8.0]. The uptake of sparfloxacin by human PMN significantly decreased during phagocytosis of opsonized H. influenzae but still reached intracellular concentrations five times greater than the extracellular ones. This effect was not observed with E. coli and S. aureus.
评估了大肠杆菌、金黄色葡萄球菌和流感嗜血杆菌的吞噬作用对司帕沙星进入人多形核中性粒细胞（PMN）细胞内的影响。含有细胞内细菌的PMN和静息PMN中司帕沙星的细胞内浓度相似[细胞与细胞外比率（C/E）>8.0]。在调理过的流感嗜血杆菌吞噬过程中，人PMN对司帕沙星的摄取显著降低，但细胞内浓度仍达到细胞外浓度的五倍。在大肠杆菌和金黄色葡萄球菌吞噬过程中未观察到这种效应。
