Kikuchi A, Taira H, Tsuruta T, Hayashi M, Kataoka K
Department of Materials Science and Technology, Science University of Tokyo, Chiba, Japan.
J Biomater Sci Polym Ed. 1996;8(2):77-90. doi: 10.1163/156856296x00165.
Polyamine-brushed substrata for cell culture were designed by solvent casting of polystyrene-graft-polyamine copolymer (SA) on hydrophobically modified glass surface, and adhesion, spreading, and proliferation of bovine aortic endothelial cells (BAEC) on these substrata were evaluated. Adhesion and spreading of BAEC increased with increasing polyamine content in the copolymer. Close correlation was found between cellular spreading and subsequent cell growth; the surface inducing better spreading of adhered cells showed higher endothelial cell growth. Adhesion and spreading of BAEC were significantly influenced by fetal calf serum (FCS)-pretreatment of the SA copolymer surfaces, being increased with increasing polyamine content, whereas on bovine serum albumin (BSA)-preabsorbed surfaces, BAEC adhesion was considerably prevented and eventually no cell spreading was observed. Then, adsorption of cell-adhesion proteins, fibronectin (FN), and vitronectin (VN), out of FCS onto SA copolymer surfaces were evaluated using enzyme immunoassay. Both FN and VN adsorption on SA copolymer surfaces were increased with increasing polyamine content in the copolymer, suggesting a crucial role of these cell-adhesion proteins in BAEC adhesion and subsequent growth behavior on SA copolymer surfaces.
通过在疏水改性的玻璃表面浇铸聚苯乙烯接枝多胺共聚物（SA）来设计用于细胞培养的多胺刷涂层基质，并评估牛主动脉内皮细胞（BAEC）在这些基质上的黏附、铺展和增殖情况。BAEC的黏附和铺展随着共聚物中多胺含量的增加而增加。发现细胞铺展与随后的细胞生长之间存在密切相关性；诱导黏附细胞更好铺展的表面显示出更高的内皮细胞生长。SA共聚物表面经胎牛血清（FCS）预处理对BAEC的黏附和铺展有显著影响，随着多胺含量的增加而增加，而在牛血清白蛋白（BSA）预吸附的表面上，BAEC的黏附受到显著抑制，最终未观察到细胞铺展。然后，使用酶免疫测定法评估FCS中的细胞黏附蛋白纤连蛋白（FN）和玻连蛋白（VN）在SA共聚物表面的吸附情况。SA共聚物表面上FN和VN的吸附均随着共聚物中多胺含量的增加而增加，表明这些细胞黏附蛋白在BAEC在SA共聚物表面的黏附及随后的生长行为中起关键作用。
