Cuvelier A, Bourguignon J, Muir J F, Martin J P, Sesboüé R
INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, France.
J Immunoassay. 1996 Nov;17(4):371-82. doi: 10.1080/01971529608005799.
The first step of sandwich ELISA, namely adsorption of antibodies to plastic microtiter plates, was studied as a function of the pH of the coating buffer. Coating efficiency was assessed in terms of maximum signal (absorbance) observed in ELISA and also estimated by measuring the amount of functional antibodies adsorbed to the plate. While goat antibodies displayed better results after coating with acetate pH 5 buffer, rabbit IgGs generally worked well at pH 7.4. On average, the classical carbonate pH 9.6 buffer was only 50% as efficient.
夹心酶联免疫吸附测定法（ELISA）的第一步，即将抗体吸附到塑料微量滴定板上，作为包被缓冲液pH值的函数进行了研究。包被效率根据ELISA中观察到的最大信号（吸光度）进行评估，也通过测量吸附到板上的功能性抗体的量来估计。虽然用pH 5的醋酸盐缓冲液包被后山羊抗体显示出更好的结果，但兔免疫球蛋白（IgGs）通常在pH 7.4时效果良好。平均而言，经典的pH 9.6碳酸盐缓冲液的效率仅为50%。
