Dmitriev Alexander D, Tarakanova Julia N, Yakovleva Dinora A, Dmitriev Dmitriy A, Phartooshnaya Olga V, Kolyaskina Galina I, Massino Yulia S, Borisova Olga V, Segal Olga L, Smirnova Maria B, Ulanova Tatiana I, Lavrov Viacheslav F
Mechnikov Research Institute of Vaccines and Sera, RAMS, O. Andzhaparidze Virology Unit, Moscow, Russia.
J Immunoassay Immunochem. 2013;34(4):414-37. doi: 10.1080/15321819.2013.764894.
This article reexamines some opinions concerning pH requirements for optimal immobilization of monoclonal antibodies (mAbs) by passive adsorption in antigen capture ELISA. It was discovered that substitution of "classical" sodium phosphate (pH 7.5) and carbonate (pH 9.5) coating solutions by acid (pH 2.8) buffers maximized antigen capture 4 out of 10 different tested anti-HBsAg mAbs, resulting in a 1.5-2.5 increase of binding curve coefficients. By measuring both mAbs amounts and functionality, the enhancement effect was attributed to the better preservation of solid phase antibodies activity.
本文重新审视了一些关于在抗原捕获ELISA中通过被动吸附实现单克隆抗体（mAb）最佳固定化所需pH值的观点。研究发现，用酸性（pH 2.8）缓冲液替代“经典的”磷酸钠（pH 7.5）和碳酸盐（pH 9.5）包被溶液，在10种不同测试的抗HBsAg单克隆抗体中有4种实现了最大抗原捕获，使结合曲线系数提高了1.5至2.5倍。通过测量单克隆抗体的数量和功能，这种增强效应归因于固相抗体活性的更好保存。
