Túri S, Németh I, Torkos A, Sághy L, Varga I, Matkovics B, Nagy J
Department of Paediatrics, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Free Radic Biol Med. 1997;22(1-2):161-8. doi: 10.1016/s0891-5849(96)00284-5.
The changes in red blood cell (RBC) lipid peroxidation [measured via the malonyl dialdehyde (MDA) concentration], reduced (GSH), and oxidized glutathione (GSSG) levels, hemoglobin (Hb) oxidation and antioxidant enzyme [catalase (Cat), glutathione peroxidase, and superoxide dismutase (SOD)] activities were studied in 45 pediatric patients with various glomerular diseases [minimal change nephrotic syndrome (MCNS) in relapse or in remission, lupus nephropathy (SLE), poststreptococcal glomerulonephritis (APSGN), IgA nephropathy (IGA gn)], and in 20 adult patients with IGA gn and also in 15 pediatric and 14 adult controls. The in vitro effects of hydrogen peroxide [acetyl phenylhydrazine (APH) test] on the GSH and Hb metabolisms were likewise investigated. There was an increased oxidative stress in MCNS with relapse, IGA gn, SLE gn, and APSGN, which could be detected in the GSH and Hb oxidation and in the lipid peroxidation on the peripheral RBC-s. The RBC SOD and Cat activities were significantly lower in all patients than in the controls. The RBC GSSG level was significantly elevated in all patients, with the exception of MCNS in remission. This stimulated a compensatory GSH production in MCNS with relapse and in IGA gn, but not in SLE or APSGN. The regeneration of GSH from GSSG was reduced in MCNS with relapse, SLE, and IGA gn, but not in APSGN. In remission, the GSH-GSSG redox system normalizes, but in vitro the APH test stimulates an intensive Hb oxidation. In conclusion, there is a correlation between the presence of active glomerular disease and the evidence of oxidative changes in the various parameters measured in peripheral RBCs.
研究了45例患有各种肾小球疾病的儿科患者[复发或缓解期的微小病变肾病综合征（MCNS）、狼疮性肾炎（SLE）、链球菌感染后肾小球肾炎（APSGN）、IgA肾病（IGA gn）]、20例患有IGA gn的成年患者以及15例儿科对照和14例成年对照的红细胞（RBC）脂质过氧化[通过丙二醛（MDA）浓度测量]、还原型（GSH）和氧化型谷胱甘肽（GSSG）水平、血红蛋白（Hb）氧化以及抗氧化酶[过氧化氢酶（Cat）、谷胱甘肽过氧化物酶和超氧化物歧化酶（SOD）]活性的变化。同样研究了过氧化氢[乙酰苯肼（APH）试验]对GSH和Hb代谢的体外影响。复发期的MCNS、IGA gn、SLE gn和APSGN存在氧化应激增加，这可在外周RBC的GSH和Hb氧化以及脂质过氧化中检测到。所有患者的RBC SOD和Cat活性均显著低于对照组。除缓解期的MCNS外，所有患者的RBC GSSG水平均显著升高。这刺激了复发期的MCNS和IGA gn中GSH的代偿性产生，但在SLE或APSGN中未出现。复发期的MCNS、SLE和IGA gn中，GSSG再生GSH的能力降低，但APSGN中未降低。缓解期，GSH - GSSG氧化还原系统恢复正常，但体外APH试验会刺激强烈的Hb氧化。总之，活动性肾小球疾病的存在与外周RBC中测量的各种参数的氧化变化证据之间存在相关性。
