Vizzi E, Ferraro D, Cascio A, Di Stefano R, Arista S
Dipartimento di Igiene e Microbiologia, Università di Palermo, Italy.
Res Virol. 1996 Nov-Dec;147(6):333-9. doi: 10.1016/s0923-2516(97)85125-4.
To examine the role of enteric adenoviruses (Ad40 and Ad41) in children with acute gastroenteritis, we evaluated 273 children with diarrhoea and 137 without enteric symptoms in Palermo, Italy, during an 8-month period. Stools were tested by two home-made monoclonal-based ELISAs to detected genus-specific adenovirus antigen and to type Ad40 and Ad41. Twenty-five samples (6.1%) were found to contain adenovirus, 18 of which were grown in Graham 293 and in HEp-2 cells. Ad40 and Ad41 were detected in 2.6% of children with diarrhoea and in none in the control group, while non-enteric adenoviruses were obtained from both patients (3.2%) and controls (6.5%). Samples containing Ad40 and Ad41 were positive by the virus isolation procedure in Graham and in HEp-2 cells, showing no distinct growth pattern in these cell lines. The evaluation of a latex agglutination test (Adenolex) and of a commercial ELISA (Adenoclone), respectively available for the detection of genus adenovirus antigen and for the typing of Ad40 and Ad41 suggests that both tests enable the identification of enteric adenoviruses in stool specimens, giving results comparable to our ELISAs.
为了研究肠道腺病毒（Ad40和Ad41）在急性胃肠炎患儿中的作用，我们在意大利巴勒莫对273名腹泻儿童和137名无肠道症状的儿童进行了为期8个月的评估。通过两种自制的基于单克隆抗体的酶联免疫吸附测定法（ELISA）检测粪便，以检测属特异性腺病毒抗原并对Ad40和Ad41进行分型。发现25份样本（6.1%）含有腺病毒，其中18份在格雷厄姆293细胞和人胚肾293细胞（HEp-2细胞）中培养。腹泻儿童中2.6%检测到Ad40和Ad41，而对照组未检测到，非肠道腺病毒在患者（3.2%）和对照组（6.5%）中均有检出。含有Ad40和Ad41的样本在格雷厄姆细胞和HEp-2细胞中通过病毒分离程序呈阳性，在这些细胞系中未显示出明显的生长模式。分别用于检测腺病毒属抗原以及对Ad40和Ad41进行分型的乳胶凝集试验（Adenolex）和商业ELISA（Adenoclone）的评估表明，这两种检测方法都能够识别粪便标本中的肠道腺病毒，其结果与我们的ELISA相当。
