Johansson M E, Uhnoo I, Svensson L, Pettersson C A, Wadell G
J Med Virol. 1985 Sep;17(1):19-27. doi: 10.1002/jmv.1890170104.
An enzyme-linked immunosorbent assay (ELISA) for direct detection of enteric adenovirus 41 (Ad41) in stool specimens was developed and compared with an Ad40-specific ELISA described previously [Johansson et al, 1980]. Rabbit antiserum to Ad41 was obtained by immunization with purified virions. To eliminate genus-specific reactivity the serum was passed through an immunosorbent column containing soluble adenovirus components of members of subgenera A to E. The anti-Ad41 serum still displayed high reactivity against Ad40 and had to be immunoabsorbed with soluble virus components of Ad40 to be rendered type-specific. The absorbed antiserum was used in an indirect ELISA and proved to be specific for Ad41. No heterotypic reactivity against Ad40 or Ad1 through Ad35 was found. The Ad41-specific ELISA proved to be of equal sensitivity to electron microscopy. The type-specific ELISAs for Ad40 and Ad41 were evaluated by testing 76 stool specimens containing enteric adenoviruses originating from England and Scandinavia. All specimens could be typed--41 (54%) as Ad40 and 35 (46%) as Ad41. These results were confirmed by DNA restriction site analysis. The type-specific ELISA proved to be a specific, sensitive, and a rapid technique for detection of Ad41 and allowed clear-cut discrimination from Ad40 in clinical specimens.
开发了一种用于直接检测粪便标本中肠道腺病毒41型（Ad41）的酶联免疫吸附测定（ELISA），并将其与先前描述的Ad40特异性ELISA进行比较[约翰松等人，1980年]。通过用纯化的病毒粒子免疫获得兔抗Ad41血清。为了消除属特异性反应性，将血清通过含有A至E亚属成员的可溶性腺病毒成分的免疫吸附柱。抗Ad41血清对Ad40仍显示出高反应性，必须用Ad40的可溶性病毒成分进行免疫吸附才能使其具有型特异性。吸附后的抗血清用于间接ELISA，证明对Ad41具有特异性。未发现对Ad40或Ad1至Ad35的异型反应性。Ad41特异性ELISA的敏感性与电子显微镜相当。通过检测76份来自英格兰和斯堪的纳维亚半岛的含有肠道腺病毒的粪便标本，对Ad40和Ad41的型特异性ELISA进行了评估。所有标本都可以分型——41份（54%）为Ad40，35份（46%）为Ad41。这些结果通过DNA限制性酶切位点分析得到证实。型特异性ELISA被证明是一种检测Ad41的特异性、敏感性和快速的技术，并且能够在临床标本中清晰地区分Ad40。
