Gravance C G, Liu I K, Davis R O, Hughes J P, Casey P J
Research Centre in Reproductive Medicine, University of Auckland, New Zealand.
J Reprod Fertil. 1996 Sep;108(1):41-6. doi: 10.1530/jrf.0.1080041.
The heads of stallion spermatozoa were analysed by computer automated sperm head morphometry and the morphometric values of the major subpopulations of sperm heads were assessed. The criteria for normal dimensions of stallion sperm heads are proposed based on the analysis of these measurements. Semen samples were collected from 10 fertile and 10 subfertile stallions, processed by a standard method, smeared onto microscope slides and stained using haematoxylin. At least 200 properly digitized sperm heads were analysed from each stallion. The measurements for length, width, area, perimeter and width/length were recorded for each stallion. All sperm head measurements were placed in a statistical database and multivariate cluster analysis performed. Mean measurements for all parameters of the major clusters of fertile and subfertile stallions were compared by analysis of variance. The ranges of the values of the major clusters of fertile stallions were applied to all stallions to determine the percentage of normal sperm heads for each stallion. The mean values for length, width, area and perimeter in the major cluster of sperm head dimensions of fertile stallions were significantly different from those of the subfertile stallions (P < 0.001). The range of values of the major cluster of fertile stallions was length = 4.9-5.7 microns, width = 2.5-3.0 microns, width/length = 0.45-0.59, area = 10.3-12.1 microns2, and perimeter = 12.9-14.2 microns. On the basis of these values, a significantly (P < 0.001) higher percentage of normal sperm heads were found in the fertile group than in the subfertile group of stallions (52% versus 19%).
通过计算机自动精子头部形态测量法对种公马精子头部进行分析，并评估精子头部主要亚群的形态测量值。基于这些测量结果分析，提出了种公马精子头部正常尺寸的标准。从10匹生育能力正常和10匹生育能力低下的种公马采集精液样本，采用标准方法进行处理，涂抹在载玻片上并用苏木精染色。每匹种公马至少分析200个数字化良好的精子头部。记录每匹种公马精子头部的长度、宽度、面积、周长和宽长比测量值。将所有精子头部测量值录入统计数据库并进行多元聚类分析。通过方差分析比较生育能力正常和生育能力低下种公马主要聚类所有参数的平均测量值。将生育能力正常种公马主要聚类的值范围应用于所有种公马，以确定每匹种公马正常精子头部的百分比。生育能力正常种公马精子头部尺寸主要聚类的长度、宽度、面积和周长平均值与生育能力低下种公马的显著不同（P < 0.001）。生育能力正常种公马主要聚类的值范围为：长度 = 4.9 - 5.7微米，宽度 = 2.5 - 3.0微米，宽长比 = 0.45 - 0.59，面积 = 范围为10.3 - 12.1平方微米，周长 = 12.9 - 14.2微米。基于这些值，发现生育能力正常的种公马组中正常精子头部的百分比显著（P < 0.001）高于生育能力低下的种公马组（52% 对19%）。
