Casey P J, Gravance C G, Davis R O, Chabot D D, Liu I K
Department of Obstetrics and Gynecology, Research Centre in Reproductive Medicine, National Women's Hospital, University of Auckland, Auckland, New Zealand.
Theriogenology. 1997 Jan 15;47(2):575-82. doi: 10.1016/s0093-691x(97)00015-0.
Gross morphological evaluation of stallion spermatozoa is of clinical value in assessing male fertility in the horse. While of value, methods of subjective sperm classification yield highly variable results. Recent development of computer-assisted sperm morphometry analysis (ASMA) technology has allowed for the objective analysis of sperm head morphometry. In the current study, ASMA was employed to determine morphometric differences in sperm head dimensions between fertile and subfertile stallions. At least 200 spermatozoa from each of 10 fertile and 10 subfertile stallions were analyzed by a commercial ASMA instrument. The mean measurements for length, width, area, perimeter, and width/length for each stallion were recorded and group means compared by a two-sample t-test. The mean measurements for length, area and perimeter were significantly larger in the subfertile than the fertile group (5.77 microm vs 5.33 microm, 12.66 microm vs 11.37 microm and 14.59 microm vs 13.64 microm, respectively). The width of sperm heads from stallions in the subfertile group also tended to be larger than those of fertile stallions. The data suggest that differences in the dimensions of sperm heads may exist between fertile and subfertile stallions.
对种马精子进行大体形态学评估，在评估公马生育能力方面具有临床价值。虽然有价值，但主观的精子分类方法得出的结果差异很大。计算机辅助精子形态测量分析（ASMA）技术的最新发展使得对精子头部形态进行客观分析成为可能。在本研究中，采用ASMA来确定可育和亚可育种马精子头部尺寸的形态测量差异。使用商用ASMA仪器对10匹可育种马和10匹亚可育种马中的每一匹至少200个精子进行分析。记录每匹种马的长度、宽度、面积、周长和宽/长的平均测量值，并通过双样本t检验比较组均值。亚可育组的长度、面积和周长的平均测量值显著大于可育组（分别为5.77微米对5.33微米、12.66微米对11.37微米和14.59微米对13.64微米）。亚可育组种马的精子头部宽度也往往大于可育种马。数据表明，可育和亚可育种马之间精子头部尺寸可能存在差异。
