Stoddart N R, Wild A E, Fleming T P
Department of Biology, School of Biological Sciences, University of Southampton, UK.
J Reprod Fertil. 1996 Sep;108(1):47-53. doi: 10.1530/jrf.0.1080047.
Previous reports have demonstrated that culture of mouse preimplantation embryos at high density stimulates their rate of development. The molecular basis of this phenomenon was investigated. Culture of embryos from the four-cell stage at high density in normal medium, or at low density either in embryo-conditioned medium or medium containing platelet-activating factor (PAF), significantly advanced the timing of compaction, initiation of cavitation and/or completion of zona hatching, and also increased the number of cells in blastocysts. In contrast, Lyso-PAF, an inactive metabolite of PAF, and Enantio-PAF, an enantiomer of PAF, did not have a stimulatory effect at low embryo density, but did not inhibit the stimulation of development at high embryo density. The stimulatory effect of culture at high density was inhibited in the presence of either CV-3988 or SDZ 64-412, two structurally distinct competitive PAF-receptor antagonists, while the development rate at low density was not affected. We conclude that an embryo-derived factor related to PAF is secreted by blastomeres during in vitro culture and acts in a receptor-mediated manner to stimulate the rate of development.
先前的报道表明，高密度培养小鼠植入前胚胎可促进其发育速度。对这一现象的分子基础进行了研究。在正常培养基中高密度培养四细胞期胚胎，或在胚胎条件培养基或含血小板活化因子（PAF）的培养基中低密度培养，均显著提前了致密化时间、囊腔形成起始时间和/或透明带孵化完成时间，并且还增加了囊胚中的细胞数量。相比之下，PAF的无活性代谢产物溶血PAF和PAF的对映体对映体PAF在低胚胎密度时没有刺激作用，但在高胚胎密度时不抑制发育刺激。在两种结构不同的竞争性PAF受体拮抗剂CV-3988或SDZ 64-412存在的情况下，高密度培养的刺激作用受到抑制，而低密度时的发育速度不受影响。我们得出结论，在体外培养过程中，卵裂球分泌一种与PAF相关的胚胎衍生因子，该因子以受体介导的方式发挥作用，刺激发育速度。
