Gamboa P M, Sanz M L, de la Cuesta C G, García B E, Castillo J G, Oehling A
Departamento de Alergología e Inmunología Clínica, Clínica Universitaria, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
J Investig Allergol Clin Immunol. 1991 Apr;1(2):113-21.
The study was carried out on venous blood from 67 patients with seasonal rhinoconjunctivitis caused by sensitization to grass pollen and 30 control individuals. Total IgE determination, antigen-specific histamine release test against two concentrations of Phleum pratense, and quantification of beta 2-adrenergic receptor numbers in lymphocyte membrane of peripheral blood were done on all patients. Those pollinic patients who were asymptomatic at the time of the study had 500.07 +/- 237.27 receptors/cell; no significant differences were established with the control group, with 541.53 +/- 123.63 receptors/cell. However, both the control group and asymptomatic patients had receptor numbers which were significantly higher than those of symptomatic pollinic patients, with 376.81 +/- 158.65 receptors/cell (p < 0.01 and p < 0.05, respectively). The average decrease in number of receptors in symptomatic pollinic patients was 30.42% in relation to controls and 24.65% in relation to asymptomatic patients. Within the subgroup of pollinic patients, studied both in and out of season, the number of beta 2-adrenergic receptors had an average decrease of 13.22% during pollination, with 363.7 receptors/cell. Once the pollination season was over, this figure increased to 419.1 receptors/cell, establishing significant differences with p < 0.025. The number of beta 2-adrenergic receptors did not correlate with total seric IgE figures or with antigen-specific histamine release. These data indicate that the decrease of these receptors does not constitute the causal factor of atopic diseases; it seems more likely to be a consequence of the same.
该研究对67例因对草花粉过敏而患有季节性鼻结膜炎的患者以及30名对照个体的静脉血进行了检测。对所有患者进行了总IgE测定、针对两种浓度的早熟禾的抗原特异性组胺释放试验以及外周血淋巴细胞膜中β2 - 肾上腺素能受体数量的定量分析。在研究时无症状的花粉症患者每细胞有500.07±237.27个受体；与对照组（每细胞541.53±123.63个受体）相比，未发现显著差异。然而，对照组和无症状患者的受体数量均显著高于有症状的花粉症患者（每细胞376.81±158.65个受体，分别为p<0.01和p<0.05）。有症状的花粉症患者受体数量相对于对照组平均减少30.42%，相对于无症状患者减少24.65%。在花粉症患者亚组中，无论在花粉季节内还是外进行研究，β2 - 肾上腺素能受体数量在授粉期间平均减少13.22%，为每细胞363.7个受体。一旦授粉季节结束，该数字增加到每细胞419.1个受体，p<0.025，差异显著。β2 - 肾上腺素能受体数量与血清总IgE值或抗原特异性组胺释放均无相关性。这些数据表明，这些受体的减少并非特应性疾病的致病因素；更有可能是其结果。
