过敏反应过程中多形核血白细胞受刺激化学发光的特异性抑制机制。
The mechanism of specific inhibition of stimulated chemiluminescence of polymorphonuclear blood leukocytes in allergic processes.
作者信息
Pytsky V I, Filatov O Y
机构信息
Department of General Pathology, Medicobiological Faculty, Russian Medical University, Moscow, Russia.
出版信息
J Investig Allergol Clin Immunol. 1996 Sep-Oct;6(5):320-3.
In the first part of our work (1) it was found that the cultivation of blood of sensitized people and animals with specific allergen (AI) caused the phenomenon of specific inhibition stimulated luminol-dependent chemiluminescence of leukocytes (PhSISCL). In the current study the mechanism of this inhibition was investigated. It was revealed that PhSISCL resulted from the direct influence of AI on polymorphonuclear leukocytes (PMNL). The activity of NADPH-dependent oxidase was established by the Wymann M. et al. method (2). It was determined that the values of chemiluminescence (CL) for the cultivation of sensitized rabbit blood with specific AI did not differ from the control. This demonstrated that the activity of NADPH-oxidase was not inhibited. On these grounds it is possible to assume that PhSISCL is connected to either the inhibition of myeloperoxidase (MPO) activity or to a release of this ferment from PMNL during cultivation. The latter was specifically investigated and was not confirmed.
在我们工作的第一部分（1）中发现，用特定变应原（AI）培养致敏人和动物的血液会引发特异性抑制现象，即刺激白细胞的鲁米诺依赖性化学发光（PhSISCL）。在当前研究中，对这种抑制的机制进行了研究。结果表明，PhSISCL是由AI对多形核白细胞（PMNL）的直接影响所致。通过Wymann M.等人的方法（2）确定了NADPH依赖性氧化酶的活性。经测定，用特定AI培养致敏兔血液时的化学发光（CL）值与对照组无差异。这表明NADPH氧化酶的活性未受抑制。基于这些理由，可以假定PhSISCL要么与髓过氧化物酶（MPO）活性的抑制有关，要么与培养过程中该酶从PMNL的释放有关。对后者进行了专门研究，但未得到证实。
Zotero 插件