Bailly S, Israël N, Fay M, Gougerot-Pocidalo M A, Duff G W
DBMS/BRCE-INSERM U244, CENG, Grenoble, France.
Mol Immunol. 1996 Aug;33(11-12):999-1006. doi: 10.1016/s0161-5890(96)00042-9.
Recently, we characterized a polymorphism within IL-1 alpha intron 6 as a variable number of a 46 bp tandem repeat (ranging from 5 to 18 repeats). We now analyse whether this polymorphism could play a role in IL-1 alpha gene regulation. We have found that reporter gene expression driven by the IL-1 alpha promoter or a heterologous promoter was decreased by increasing numbers of the repeat sequence corresponding to the most frequent alleles seen in the human population. Furthermore, we showed that the transcription factor Sp1 can bind to the 46 bp sequence. Finally, we were unable to show a statistically-significant relation between in vitro IL-1 alpha production and the number of repeats although there was a clear trend towards an inverse relation. Taken together, these results are consistent with a negative regulatory role for IL-1 alpha intron 6 repeat sequence on IL-1 alpha basal gene transcription.
最近，我们将白细胞介素-1α（IL-1α）内含子6中的一种多态性特征化为46 bp串联重复序列的可变数量（范围为5至18个重复）。我们现在分析这种多态性是否可能在IL-1α基因调控中发挥作用。我们发现，由IL-1α启动子或异源启动子驱动的报告基因表达会随着对应于人群中最常见等位基因的重复序列数量增加而降低。此外，我们表明转录因子Sp1可以结合到46 bp序列上。最后，尽管存在明显的负相关趋势，但我们未能显示出体外IL-1α产生与重复序列数量之间具有统计学意义的关系。综上所述，这些结果与IL-1α内含子6重复序列对IL-1α基础基因转录的负调控作用一致。
