Tabarowski Z, Gibson-Berry K, Felten S Y
Jagiellonian University, Department of Animal Physiology, Cracow, Poland.
Acta Histochem. 1996 Nov;98(4):453-7. doi: 10.1016/S0065-1281(96)80013-4.
The innervation of bone marrow from femur bones of BALB/c mice was studied by means of immunohistochemistry and fluorescence histochemistry. The immunoperoxidase method with nickel amplification was applied to visualize the topographical distribution of nerve fibers using antibodies against the general neuronal marker PGP 9.5 (neuron-specific cytoplasmic protein), catecholamine synthesizing enzyme tyrosine hydroxylase (TH) and neuropeptide Y (NPY). Glyoxylic acid-induced fluorescence was also applied to demonstrate catecholamine-containing nerves. Both staining methods revealed dense innervation by fibers seen predominantly around blood vessels but also ramifying among marrow cells. Recent findings on adrenergic and peptidergic influences on marrow physiology combined with anatomical data indicate the existence of a neural modulation of hematopoiesis.
通过免疫组织化学和荧光组织化学方法研究了BALB/c小鼠股骨骨髓的神经支配。采用镍增强免疫过氧化物酶法，使用针对一般神经元标志物PGP 9.5（神经元特异性细胞质蛋白）、儿茶酚胺合成酶酪氨酸羟化酶（TH）和神经肽Y（NPY）的抗体来可视化神经纤维的拓扑分布。还应用乙醛酸诱导荧光来显示含儿茶酚胺的神经。两种染色方法均显示纤维密集支配，主要见于血管周围，但也在骨髓细胞间分支。关于肾上腺素能和肽能对骨髓生理学影响的最新研究结果与解剖学数据相结合，表明存在对造血的神经调节。
