Brown M D, Egginton S, Hudlicka O, Zhou A L
School of Sport and Exercise Sciences, University of Birmingham, UK.
Exp Physiol. 1996 Nov;81(6):1043-6. doi: 10.1113/expphysiol.1996.sp003989.
The endothelial glycocalyx layer of capillaries was stained with Ruthenium Red and examined by transmission electron microscopy in rat extensor hallucis proprius muscles after 2 or 4 days of indirect electrical stimulation via implanted electrodes. The layer appeared continuous on the luminal surface of 63% capillaries in control muscles and absent 13%. This situation was reversed in muscles stimulated for 2 or 4 days, with a continuous layer in 12 and 10% of capillaries and no glycocalyx in 44 and 58%, respectively. The greater disruption of the endothelial luminal glycocalyx in capillaries from stimulated muscles suggests that flow-related shear forces on endothelial cells could be important signals for initiation of angiogenesis in actively contracting skeletal muscle.
通过植入电极对大鼠拇长伸肌进行2天或4天的间接电刺激后，用钌红对毛细血管的内皮糖萼层进行染色，并通过透射电子显微镜检查。在对照肌肉中，该层在63%的毛细血管腔表面呈连续状态，13%缺失。在刺激2天或4天的肌肉中，这种情况发生了逆转，分别有12%和10%的毛细血管有连续层，44%和58%没有糖萼。来自受刺激肌肉的毛细血管内皮腔糖萼的更大破坏表明，作用于内皮细胞的与血流相关的剪切力可能是活跃收缩的骨骼肌中血管生成起始的重要信号。
