Key Laboratory of Resource Utilization of Agricultural Solid Waste in Gansu Province, Tianshui Normal University, South Xihe Road, Qinzhou District, Tianshui, 741000, Gansu Province, People's Republic of China.
Zhangjiachuan Country Fishery Technology Promotion Station, Tianshui City, Gansu Province, People's Republic of China.
Trop Anim Health Prod. 2023 Aug 5;55(5):287. doi: 10.1007/s11250-023-03705-1.
The tsinling lenok trout (Brachymystax Lenok tsinlingensis) is a typical land-locked cold salmon. In this study, through the acute high temperature stress (16, 24, and 26°C), samples were taken at multiple temperature points to analyze the dynamic changes of serum non-specific immune indexes and histopathological changes of tissues of tsinling lenok trout. The histopathological investigation of different studied tissues revealed an increase of histological lesions' frequency and severity with increasing water temperature. The activity of T-SOD, GSH-Px, CAT, ACP, and LZM and MDA concentration are all impacted by high temperature stress. The activities of T-SOD, GSH-Px, and ACP are significantly lower in temperatures higher than 16°C (P<0.05). However, with the increase of water temperature, MDA content increased significantly. The activities of CAT and LZM were the highest at 24°C, which were significantly higher than those at 26°C (P<0.05). The above results indicate that 24°C is a "critical high temperature point" for tsinling lenok trout under heat stress, and this temperature point may be the critical point for tsinling lenok trout to enter "damaged" from adaptive adjustment. Our results can provide a theoretical basis for the development of genetic breeding, improvement, and control measures of heat stress in tsinling lenok trout in the future.
秦岭细鳞鲑（Brachymystax lenok tsinlingensis）是一种典型的陆封冷水鲑鱼。本研究通过急性高温胁迫（16、24 和 26°C），在多个温度点取样，分析了秦岭细鳞鲑血清非特异性免疫指标的动态变化和组织的组织病理学变化。对不同研究组织的组织病理学研究表明，随着水温的升高，组织病变的频率和严重程度增加。高温胁迫对 T-SOD、GSH-Px、CAT、ACP 和 LZM 的活性以及 MDA 浓度均有影响。在 16°C 以上的温度下，T-SOD、GSH-Px 和 ACP 的活性显著降低（P<0.05）。然而，随着水温的升高，MDA 含量显著增加。CAT 和 LZM 的活性在 24°C 时最高，明显高于 26°C（P<0.05）。以上结果表明，24°C 是秦岭细鳞鲑在热应激下的“临界高温点”，该温度点可能是秦岭细鳞鲑从适应调整进入“受损”的临界点。我们的研究结果可为未来秦岭细鳞鲑遗传育种、热应激改良和控制措施的发展提供理论基础。
