Schimmelpfennig B, Witmer R
Ophthalmologica. 1977;175(5):275-80. doi: 10.1159/000308669.
Seven human corneae were preserved 1 or 2 weeks in a continuous tissue culture medium at +37 degrees C. 200 microgram/ml gentamycin and 2.5 microgram/ml amphotericin B were added, and no infection was noted after 2 weeks. Histological and electronmicroscopical findings presented a picture of well-kept cellular and subcellular structures. Comparable electron-optical examinations taken on seven corneae kept at +4 degrees C in a modified medium 199 showed the beginning of endothelial necrobiosis.
七个人类角膜在37摄氏度的连续组织培养基中保存1至2周。添加了200微克/毫升庆大霉素和2.5微克/毫升两性霉素B，两周后未发现感染。组织学和电子显微镜检查结果显示细胞和亚细胞结构保存良好。对在改良培养基199中于4摄氏度保存的七个角膜进行的类似电子光学检查显示内皮细胞坏死开始。
