Wu H K, Sheu S J
Department of Chemistry, National Taiwan Normal University, Taipei.
J Chromatogr A. 1996 Nov 8;753(1):139-46. doi: 10.1016/s0021-9673(96)00525-0.
A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoyla biflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 micrograms/ml. The relative standard deviations of migration times were less than 1.43% (n = 6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.
已开发出一种结合毛细管区带电泳（CZE）和胶束电动色谱（MEKC）技术的方法，用于分离总共8种芍药成分。采用CZE法测定丹皮酚、氧化芍药苷、苯甲酸、五倍子酰葡萄糖和没食子酸的含量，并应用基于胆酸钠的MEKC技术分析芍药苷、芍药内酯苷、苯甲酰芍药苷、丹皮酚和氧化芍药苷。通常在两个数量级的浓度范围内获得线性关系，这些化合物的检测限在2.6 - 23.7微克/毫升范围内。迁移时间的相对标准偏差小于1.43%（n = 6）。用该方法可轻松测定芍药样品乙醇 - 水提取物中芍药成分的含量。
