Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China.
J Zhejiang Univ Sci B. 2011 Mar;12(3):193-200. doi: 10.1631/jzus.B1000135.
Central composite design (CCD), together with multiple linear regression, was successfully used to optimize the electrophoretic buffer system of micellar electrokinetic capillary chromatography (MEKC) for the determination of albiflorin, paeoniflorin, liquiritin, and glycyrrhizic acid in the traditional Chinese medicine (TCM) prescription, Yangwei granule. Concentrations of sodium deoxycholate (SDC) and borate, and proportions of ammonia, acetonitrile, and methanol were optimized. The total resolutions of peaks between the analytes and their adjacent peaks in real samples were integrated into the evaluation index of separation efficiency. The optimum electrophoretic buffer contained 80 mmol/L SDC, 20 mmol/L borate, 5% (v/v) methanol, 0.5% (v/v) ammonia, and 5% (v/v) acetonitrile. The correlation coefficients (R(2)) between the peak areas and the corresponding concentrations of analytes were greater than 0.9956. The limits of detection (LODs) (S/N=3) of the analytes were 0.97-4.00 μg/ml. The results indicate the superiority of CCD in optimizing the separation conditions of complex samples such as TCM prescriptions.
中心组合设计（CCD）与多元线性回归相结合，成功地用于优化胶束电动毛细管色谱（MEKC）的电泳缓冲体系，以测定中药（TCM）方剂养胃颗粒中的白花前胡素、芍药苷、甘草苷和甘草酸。优化了脱氧胆酸钠（SDC）和硼酸盐的浓度以及氨、乙腈和甲醇的比例。将真实样品中分析物与其相邻峰之间的总分辨率纳入分离效率评价指标。最佳电泳缓冲液包含 80mmol/L SDC、20mmol/L 硼酸盐、5%（v/v）甲醇、0.5%（v/v）氨和 5%（v/v）乙腈。分析物的峰面积与相应浓度之间的相关系数（R²）大于 0.9956。分析物的检测限（LOD）（S/N=3）为 0.97-4.00μg/ml。结果表明 CCD 在优化复杂样品（如 TCM 方剂）分离条件方面具有优越性。
