Arienti H M, Guignard S I, Rinaldi D E, Elbarcha O C
Laboratorio de Parasitología, Hospital Rawson, Córdoba, Argentina.
Bol Oficina Sanit Panam. 1996 Sep;121(3):221-7.
The sera of 176 patients with epidemiologic antecedents or radiologic and clinical signs of hydatidosis were tested by counter-immunoelectrophoresis (CIE) and enzyme-linked immunoassay (ELISA). A semipurified antigen from cysts of human origin were used for both techniques. The results were compared with those obtained from complementary radiologic studies and were confirmed by examination of excised cysts. Biopsy confirmed the diagnosis of hydatidosis in 65 patients (37%) and revealed the presence of other diseases in the remaining 111 (63%). Of the original 176 patients, 36 (20.4%) were positive by CIE and 62 (35.2%) by ELISA. Both techniques showed an excellent correlation with postsurgical diagnosis; neither produced any false positives, and the ELISA gave false negative results for only three patients (4.6%) with cysts that were infected, infertile, or calcified to some degree. The paper describes standardization of an inexpensive and easy-to-use microELISA.
对176例有包虫病流行病学史或有影像学及临床症状的患者血清进行了对流免疫电泳（CIE）和酶联免疫吸附测定（ELISA）检测。两种技术均使用了来自人源囊肿的半纯化抗原。将结果与补充影像学研究结果进行比较，并通过对切除囊肿的检查进行确认。活检确诊65例（37%）患者患有包虫病，其余111例（63%）显示存在其他疾病。在最初的176例患者中，CIE检测阳性36例（20.4%），ELISA检测阳性62例（35.2%）。两种技术与术后诊断均显示出极好的相关性；均未产生任何假阳性结果，ELISA仅对3例（4.6%）囊肿有感染、不育或某种程度钙化的患者给出假阴性结果。本文描述了一种廉价且易于使用的微量ELISA的标准化方法。
