Viviente E, Bujía J, Torregrosa C, Osete J M, Medina A, Sprekelsen C, Wilmes E
Servicio de ORL, Hospital General Universitario, Murcia.
Acta Otorrinolaringol Esp. 1996 Jul-Aug;47(4):263-7.
Transplantation of preserved cartilage has an important role in reconstructive surgery. Opinions vary with regard to the performance of cryopreserved cartilage. We studied the functional state of chondrocytes after cryopreservation. Cellular survival was studied using the trypan blue dye exclusion test, a functional assay for cell adhesion, and transmission electron microscopy. Most chondrocytes were irreversibly damaged by cryopreservation and the cartilage could not originate new cartilage. Therefore, cryopreserved cartilage tends to generate fibrosis and resorption and is not practical for reconstructing skeletal parts exposed to mechanical stress.
保存软骨的移植在重建手术中具有重要作用。关于冷冻保存软骨的性能，观点不一。我们研究了冷冻保存后软骨细胞的功能状态。使用台盼蓝染料排除试验、细胞黏附功能测定和透射电子显微镜对细胞存活情况进行了研究。大多数软骨细胞因冷冻保存而受到不可逆损伤，且软骨无法生成新的软骨。因此，冷冻保存的软骨容易发生纤维化和吸收，对于重建承受机械应力的骨骼部位并不实用。
