十二指肠贾第虫细胞骨架的深蚀刻研究。
A deep-etch study of the cytoskeleton of Giardia duodenalis.
作者信息
Kattenbach W M, Diniz Junior J A, Benchimol M, de Souza W
机构信息
Laboratório de Ultraestrutura Celular Hertha Meyer, Univeridade Federal do Rio de Janeiro, Brazil.
出版信息
Biol Cell. 1996;86(2-3):161-6. doi: 10.1016/0248-4900(96)84780-0.
The quick-freeze, freeze-fracture, deep-etching and rotary replication techniques were used to analyze the structural organization of Giardia duodenalis. The surface of the flagella was rugous, in contrast to the cell body surface which was smooth. The ventral region was characterized in more detail, exposing the layer of sub-pellicular microtubules bonded to the microribbon sheet which appears as an open flat helicoid structure where the two free ends overlap and adhere close to the frontal part of the protozoan. The microribbon appears as 18-nm thick parallel (35 nm interval) filaments connected by short bridges. A flattened structure with a highly organized array of particles was seen close to the microribbons.
采用快速冷冻、冷冻断裂、深度蚀刻和旋转复制技术分析了十二指肠贾第虫的结构组织。鞭毛表面粗糙，而细胞体表面光滑。对腹侧区域进行了更详细的表征，揭示了与微丝带片相连的表膜下微管层，微丝带片呈现为开放的扁平螺旋结构，其两个自由端重叠并紧贴原生动物前部。微丝带呈现为18纳米厚的平行（间隔35纳米）细丝，由短桥连接。在微丝带附近可见一个具有高度有序颗粒阵列的扁平结构。
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