Benchimol M, Kachar B, de Souza W
Departamento de Parasitologia e Biofísica Celular, Universidade Federal do Rio de Janeiro, Brasil.
Biol Cell. 1993;77(3):289-95. doi: 10.1016/s0248-4900(05)80200-x.
The quick-freezing and freeze-etching technique was used to analyse the cytoskeleton of Tritrichomonas foetus, a pathogenic protozoan of the urogenital tract of cattle. The cytoplasm presented a network of filamentous structures interacting with each other, with the surface of the hydrogenosomes and the nuclear membrane. Two nm wide filamentous structures were found in the luminal space of the Golgi complex, connecting the two faces of each cisterna. The microtubules of the pelta-axostyle system were connected by bridges 30-40 nm long and 10 nm wide, regularly spaced with an interval of 25 nm. The costa is a structure formed by a complex array of filaments and globous structures. It seems to be connected to the recurrent flagellum through a complex network formed by 15 and 10 nm wide filaments which emerge from the peripheral region of the costa and penetrate into the surface projections of the protozoan body to which the recurrent flagellum is attached. Other filaments were seen connecting the surface of these projections with the surface of the flagellum.
采用快速冷冻和冷冻蚀刻技术分析胎儿三毛滴虫的细胞骨架，胎儿三毛滴虫是牛泌尿生殖道的一种致病性原生动物。细胞质呈现出相互作用的丝状结构网络，与氢化酶体表面和核膜相连。在高尔基体复合体的管腔空间中发现了宽2纳米的丝状结构，连接着每个扁平囊的两个面。盾片-轴柱系统的微管由长30 - 40纳米、宽10纳米的桥连接，间隔25纳米规则排列。肋是由复杂的丝状和球状结构阵列形成的结构。它似乎通过一个由15纳米和10纳米宽的细丝组成的复杂网络与回鞭毛相连，这些细丝从肋的周边区域伸出并穿透到原生动物身体的表面突起，回鞭毛附着在该突起上。还可见其他细丝将这些突起的表面与鞭毛的表面相连。
