Zavil'gel'skiĭ G B, Manukhov I V, Rastorguev S M
Genetika. 1996 Aug;32(8):1148-52.
The EcoK restriction of nonmodified phage lambda.0 controlled by Escherichia coli K12 lon- cells is alleviated 10-20-fold in the presence of a hybrid plasmid containing the entire lux regulon of Vibrio fischeri. The La protease participates in degradation of the regulatory LuxR-LuxI proteins; therefore, transcription of the right lux operon begins significantly earlier than in the lon+ bacteria and is characterized by high content of the N-(3-oxohexanoyl) homoserine lactone autoinducer in medium. The effect of lux-induced alleviation of type I restriction in the lon mutants of E.coli K12 is proposed to depend on the reduction at the intracellular level of S-adenosylmethionine, one of the LuxI synthase substrates responsible for autoinducer synthesis.
由大肠杆菌K12 lon-细胞控制的未修饰噬菌体λ.0的EcoK限制在存在包含费氏弧菌完整lux操纵子的杂交质粒时减轻了10到20倍。La蛋白酶参与调节性LuxR-LuxI蛋白的降解；因此，右侧lux操纵子的转录比lon+细菌中显著更早开始，并且其特征是培养基中N-(3-氧代己酰基)高丝氨酸内酯自诱导剂含量高。有人提出，lux诱导的大肠杆菌K12 lon突变体中I型限制减轻的效应取决于细胞内S-腺苷甲硫氨酸水平的降低，S-腺苷甲硫氨酸是负责自诱导剂合成的LuxI合酶底物之一。
