PM2超螺旋DNA的紫外线照射
Ultraviolet light irradiation of PM2 superhelical DNA.
作者信息
Woodworth-Gutai M, Lebowitz J, Kato A C, Denhardt D T
出版信息
Nucleic Acids Res. 1977;4(5):1243-56. doi: 10.1093/nar/4.5.1243.
Abstract
Superhelical PM2 DNA can be photochemically modified by u.v. irradiation. The variation of S20,w with dose shows the following characteristics. There is a linear increase from 28 to 31s produced by a low dose of u.v. irradiation (4,000 ergs/mm2). A plateau in S20,w occurs between 4,000 and 10,000 ergs/mm2. The S20,w then increases when irradiation is increased to 56,000 ergs/mm2. Thymine dimers are introduced proportional to dose throughtout the range of exposure to u.v. light. Sedimentation velocity-dye titrations reveal anomolous behavior, i.e. apparent increases in superhelix density (sigma). However, the dye-buoyant density procedure showed no change in sigma under the same conditions. The most satisfactory model for the data is preferential photochemical modification of premelted (possibly hairpin) sites as a greater rate than the introduction of photoproducts into duplex sites. The origin of the anomoly in the sedimentation velocity dye titrations is still unclear.
摘要
超螺旋PM2 DNA可通过紫外线照射进行光化学修饰。沉降系数S20,w随剂量的变化呈现出以下特征。低剂量紫外线照射（4000尔格/平方毫米）会使S20,w从28s线性增加至31s。在4000至10000尔格/平方毫米之间，S20,w出现平稳期。当照射剂量增加到56000尔格/平方毫米时，S20,w会再次增加。在整个紫外线照射范围内，胸腺嘧啶二聚体的引入与剂量成正比。沉降速度-染料滴定显示出异常行为，即超螺旋密度（σ）明显增加。然而，在相同条件下，染料浮力密度法显示σ没有变化。对这些数据最令人满意的模型是，预熔解（可能是发夹状）位点的优先光化学修饰速率高于光产物引入双链位点的速率。沉降速度染料滴定中异常现象的起源仍不清楚。
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本文引用的文献
1
Equilibrium sedimentation of macromolecules and viruses in a density gradient.大分子和病毒在密度梯度中的平衡沉降
Fortschr Chem Org Naturst. 1962;20:373-422.
2
Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174.关于用噬菌体φX174的脱氧核糖核酸感染细菌原生质体的观察
Biochim Biophys Acta. 1963 Jun 25;72:290-7.
3
Unpaired bases in superhelical DNA: kinetic evidence.超螺旋DNA中的未配对碱基：动力学证据。
Nucleic Acids Res. 1974 Apr;1(4):549-58. doi: 10.1093/nar/1.4.549.
4
Ultraviolet sensitivity of the biological activity of phi-X-174 virus, single-stranded DNA, and RF DNA.φ-X-174病毒、单链DNA和双链环状复制型DNA生物活性的紫外线敏感性
Biophys J. 1967 May;7(3):267-78. doi: 10.1016/S0006-3495(67)86587-1.
5
The evaluation of standard sedimentation coefficients of sodium RNA and sodium DNA from sedimentation velocity data in concentrated NaCl and CsCl solutions.根据浓氯化钠和氯化铯溶液中的沉降速度数据评估核糖核酸钠和脱氧核糖核酸钠的标准沉降系数。
Biochim Biophys Acta. 1965 Sep 6;108(1):18-29. doi: 10.1016/0005-2787(65)90104-8.
6
Photodimer of thymine in ultraviolet-irradiated DNA: proof of structure by x-ray diffraction.
Science. 1968 Jun 28;160(3835):1451-2. doi: 10.1126/science.160.3835.1451.
7
Partial alteration of secondary structure in native superhelical DNA.天然超螺旋DNA二级结构的部分改变。
Nat New Biol. 1971 May 5;231(18):5-8.
8
DNA of bacteriophage PM2: a closed circular double-stranded molecule.噬菌体PM2的DNA：一种封闭的环状双链分子。
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164-8. doi: 10.1073/pnas.63.4.1164.
9
A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.一种用于检测和分离闭环双链DNA的染料浮力密度法：HeLa细胞中的闭环DNA
Proc Natl Acad Sci U S A. 1967 May;57(5):1514-21. doi: 10.1073/pnas.57.5.1514.
10
Effect of DNA length on the free energy of binding of an unwinding ligand to a supercoiled DNA.DNA长度对解旋配体与超螺旋DNA结合自由能的影响。
J Mol Biol. 1972 May 14;66(2):307-9. doi: 10.1016/0022-2836(72)90482-2.
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