Snella E, Pross S, Friedman H
Department of Medical Microbiology and Immunology, University of South Florida College of Medicine, Tampa 33612, USA.
Int J Immunopharmacol. 1995 Dec;17(12):1045-54. doi: 10.1016/0192-0561(95)00089-5.
The effect of delta 9-tetrahydrocannabinol, the major psychoactive component of marijuana, was investigated utilizing lymphoid cells from 2-week, 2-month, and 18-month-old mice. Previous studies have shown a differential modulation by THC related to age such that cells from adult mice could be up-regulated by THC when stimulated by their CD3 receptor. Cells from 2-week-old and 18-month-old mice were resistant to this THC-mediated enhancement. This paper questioned whether these resistant cells could be up-regulated by either addition or removal of cytokines or by exposure to supernatants derived from adult cells. IL-1, IL-4, and IL-6 modified cell proliferation in general, and their effects had some age-related differences, but these actions were independent of THC. In contrast, the THC-induced enhancement appeared to be related in part to IL-2 levels in the adult cell cultures such that when IL-2 was removed, not only did up-regulation not occur, but THC was, in fact, suppressive. Addition of IL-2 or supernatants from adult cells did lead to a modified THC-induced up-regulation of proliferation in cells from adult or 2-week-old mice. Cells from 18-month-old mice remained resistant to this modulation by THC. This did not represent a general anergy of these older cells since they did proliferate well in culture. These results demonstrate a difference in immune response to THC related to the age of the mice which correlates at least in part to IL-2 levels in 2-week-old and young adult mice. THC modulation, whether immunoenhancing or suppressing, appears to be influenced by the presence of other cell stimulators such as cytokines, and is sensitive to the timing of THC exposure relative to such stimuli.
利用2周龄、2月龄和18月龄小鼠的淋巴细胞，研究了大麻主要精神活性成分δ9-四氢大麻酚的作用。先前的研究表明，THC对年龄有不同的调节作用，成年小鼠的细胞在受到CD3受体刺激时可被THC上调。2周龄和18月龄小鼠的细胞对这种THC介导的增强作用具有抗性。本文质疑这些抗性细胞是否可以通过添加或去除细胞因子或暴露于成年细胞来源的上清液来上调。IL-1、IL-4和IL-6一般会改变细胞增殖，它们的作用存在一些与年龄相关的差异，但这些作用与THC无关。相比之下，THC诱导的增强作用似乎部分与成年细胞培养物中的IL-2水平有关，即当去除IL-2时，不仅上调不会发生，而且事实上THC具有抑制作用。添加IL-2或成年细胞的上清液确实会导致THC诱导的成年或2周龄小鼠细胞增殖上调发生改变。18月龄小鼠的细胞对THC的这种调节作用仍然具有抗性。这并不代表这些老年细胞普遍无反应，因为它们在培养中增殖良好。这些结果表明，小鼠对THC的免疫反应存在与年龄相关的差异，这至少部分与2周龄和年轻成年小鼠的IL-2水平相关。THC的调节作用，无论是免疫增强还是抑制，似乎都受到其他细胞刺激物（如细胞因子）的存在的影响，并且对THC暴露相对于此类刺激的时间敏感。
