Jank P, Shindo-Okada N, Nishimura S, Gross H J
Nucleic Acids Res. 1977 Jun;4(6):1999-2008. doi: 10.1093/nar/4.6.1999.
The major valine acceptor tRNA1Val from rabbit liver was purified and its nucleotide sequence determined by in vitro [32P] - labeling with T4 phage induced polynucleotide kinase and finger-printing techniques. Its primary structure was found to be identical with the major valine tRNA from mouse myeloma cells. According to the wobble hypothesis this tRNA, which exclusively has an IAC anticodon, should decode the valine codons GUU, GUC and GUA only. However, this tRNA recognizes all four valine codons with a surprising preference for GUG. It is unknown whether this is due to the lack of A37 modification next to the 3' end of the anticodon IAC. The nature of the inosine-guanosine interaction remains to be clarified.
从兔肝脏中纯化出主要的缬氨酸受体tRNA1Val，并通过用T4噬菌体诱导的多核苷酸激酶进行体外[32P]标记和指纹技术确定其核苷酸序列。发现其一级结构与来自小鼠骨髓瘤细胞的主要缬氨酸tRNA相同。根据摆动假说，这种仅具有IAC反密码子的tRNA应该只能解码缬氨酸密码子GUU、GUC和GUA。然而，这种tRNA能识别所有四个缬氨酸密码子，且对GUG有惊人的偏好。尚不清楚这是否是由于反密码子IAC的3'端旁边缺乏A37修饰。肌苷 - 鸟苷相互作用的性质仍有待阐明。
