兔肝tRNA1Val：I. 一级结构与异常密码子识别
Rabbit liver tRNA1Val:I. Primary structure and unusual codon recognition.
作者信息
Jank P, Shindo-Okada N, Nishimura S, Gross H J
出版信息
Nucleic Acids Res. 1977 Jun;4(6):1999-2008. doi: 10.1093/nar/4.6.1999.
Abstract
The major valine acceptor tRNA1Val from rabbit liver was purified and its nucleotide sequence determined by in vitro [32P] - labeling with T4 phage induced polynucleotide kinase and finger-printing techniques. Its primary structure was found to be identical with the major valine tRNA from mouse myeloma cells. According to the wobble hypothesis this tRNA, which exclusively has an IAC anticodon, should decode the valine codons GUU, GUC and GUA only. However, this tRNA recognizes all four valine codons with a surprising preference for GUG. It is unknown whether this is due to the lack of A37 modification next to the 3' end of the anticodon IAC. The nature of the inosine-guanosine interaction remains to be clarified.
摘要
从兔肝脏中纯化出主要的缬氨酸受体tRNA1Val，并通过用T4噬菌体诱导的多核苷酸激酶进行体外[32P]标记和指纹技术确定其核苷酸序列。发现其一级结构与来自小鼠骨髓瘤细胞的主要缬氨酸tRNA相同。根据摆动假说，这种仅具有IAC反密码子的tRNA应该只能解码缬氨酸密码子GUU、GUC和GUA。然而，这种tRNA能识别所有四个缬氨酸密码子，且对GUG有惊人的偏好。尚不清楚这是否是由于反密码子IAC的3'端旁边缺乏A37修饰。肌苷 - 鸟苷相互作用的性质仍有待阐明。
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本文引用的文献
1
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FEBS Lett. 1970 Dec;11(3):160-164. doi: 10.1016/0014-5793(70)80518-x.
2
Methyl-deficient mammalian transfer RNA: II. Homologous methylation in vitro of liver tRNA from normal and ethionine-fed rats: ethionine effect on 5-methyl-cytidine synthesis in vivo.甲基缺乏的哺乳动物转运RNA：II. 正常和乙硫氨酸喂养大鼠肝脏tRNA的体外同源甲基化：乙硫氨酸对体内5-甲基胞嘧啶合成的影响
Nucleic Acids Res. 1974 Oct;1(10):1259-67. doi: 10.1093/nar/1.10.1259.
3
Codon--anticodon pairing: the wobble hypothesis.密码子-反密码子配对：摆动假说
J Mol Biol. 1966 Aug;19(2):548-55. doi: 10.1016/s0022-2836(66)80022-0.
4
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
5
Isolation of mammalian transfer RNA.哺乳动物转运核糖核酸的分离
Biochim Biophys Acta. 1969 Nov 19;195(1):13-5. doi: 10.1016/0005-2787(69)90597-8.
6
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7
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8
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