黑腹果蝇缬氨酸转运核糖核酸的纯化及特性

The purification and properties of valine tRNAs of Drosophila melanogaster.

作者信息

Dunn R, Addison W R, Gillam I C, Tener G M

出版信息

Can J Biochem. 1978 Jun;56(6):618-23. doi: 10.1139/o78-093.

Abstract

The valine transfer ribonucleic acids of Drosophila melanogaster have been purified by column chromatography on BD-cellulose, Sepharose 6B, and RPC-5. Three major species were analyzed for base composition and coding properties. Valyl-tRNAVal3a binds strongly to ribosomes in the presence of GUA and to a lesser extent with GUU and GUG. Valyl-tRNAVal3b binds strongly in the presence of GUG and very poorly if at all with the other three triplets whereas valvyl-tRNAVal4, which contains inosine, binds strongly in the presence of GUU, GUC, and GUA and weakly with GUG.

摘要

黑腹果蝇的缬氨酸转移核糖核酸已通过在BD-纤维素、琼脂糖凝胶6B和RPC-5上进行柱色谱法纯化。对三种主要类型进行了碱基组成和编码特性分析。在存在鸟嘌呤核苷酸（GUA）的情况下，缬氨酰-tRNAVal3a与核糖体强烈结合，在存在鸟嘌呤尿嘧啶核苷酸（GUU）和鸟嘌呤鸟嘌呤核苷酸（GUG）时结合程度稍低。缬氨酰-tRNAVal3b在存在GUG时强烈结合，而在存在其他三个三联体时结合非常弱，而含有次黄嘌呤的缬氨酰-tRNAVal4在存在GUU、鸟嘌呤胞嘧啶核苷酸（GUC）和GUA时强烈结合，在存在GUG时结合较弱。