大鼠α-乳白蛋白信使核糖核酸的部分纯化
Partial purification of rat alpha-lactalbumin mRNA.
作者信息
Chakrabartty P K, Qasba P K
出版信息
Nucleic Acids Res. 1977 Jun;4(6):2065-74. doi: 10.1093/nar/4.6.2065.
Abstract
Alpha-lactalbumin messenger RNA was partially purified from RNA extracted from 3-5 day lactating rat mammary glands on a poly(U)-sepharose column followed by sucrose gradient centrifugation. Apha-Lactalbumin mRNA activity was assayed in wheat germ cell-free translational system by immunoprecipitation of the in vitro synthesized protein using specific antiserum prepared against purified rat alpha-lactalbumin. In the purified mRNA preparation alpha-lactalbumin mRNA activity comprised approximately 85% of the total mRNA activity.
摘要
从3 - 5日龄泌乳大鼠乳腺提取的RNA在聚（U） - 琼脂糖柱上进行部分纯化，随后进行蔗糖梯度离心，从而得到α-乳白蛋白信使核糖核酸。在小麦胚芽无细胞翻译系统中，使用针对纯化的大鼠α-乳白蛋白制备的特异性抗血清对体外合成的蛋白质进行免疫沉淀，以此来测定α-乳白蛋白信使核糖核酸的活性。在纯化的信使核糖核酸制剂中，α-乳白蛋白信使核糖核酸活性约占总信使核糖核酸活性的85%。
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