增殖性感染中腺病毒信使核糖核酸的分离与特性分析
Isolation and characterization of adenovirus messenger ribonucleic acid in productive infection.
作者信息
Lindberg U, Persson T, Philipson L
出版信息
J Virol. 1972 Nov;10(5):909-19. doi: 10.1128/JVI.10.5.909-919.1972.
Abstract
Messenger ribonucleic acid (mRNA) from cells productively infected with adenovirus type 2 was isolated by affinity chromatography on polyuridylic acid [poly (U)] bound to Sepharose. At least 90% of the polyadenylic acid [poly (A)]-containing polysomal mRNA was retained by the poly (U) Sepharose and thus separated from more than 95% of the ribosomal RNA and transfer RNA. In these experiments, 65% of the early (3 to 5 hr postinfection) and 85% of the late (14 to 16 hr postinfection) virus-specific RNA was retained by the poly (U) Sepharose. Early in the infection 18%, and late in the infection more than 95%, of the poly (A)-containing fraction, eluted from the poly (U) Sepharose with 90% formamide, was adenovirus-specific, as shown by exhaustive hybridization. Different patterns, containing several distinct species of viral mRNA, were detected early and late in the infectious cycle. No distinct viral mRNA lacking poly (A) was discovered.
摘要
通过在结合于琼脂糖珠的聚尿苷酸[聚（U）]上进行亲和层析，从被2型腺病毒有效感染的细胞中分离出信使核糖核酸（mRNA）。至少90%含聚腺苷酸[聚（A）]的多核糖体mRNA被聚（U）琼脂糖珠保留，从而与超过95%的核糖体RNA和转运RNA分离。在这些实验中，65%的早期（感染后3至5小时）和85%的晚期（感染后14至16小时）病毒特异性RNA被聚（U）琼脂糖珠保留。感染早期，从聚（U）琼脂糖珠用90%甲酰胺洗脱的含聚（A）部分的18%，以及感染后期的超过95%，经彻底杂交显示为腺病毒特异性。在感染周期的早期和晚期检测到不同的模式，包含几种不同的病毒mRNA种类。未发现缺乏聚（A）的明显病毒mRNA。
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