Nanda N, Joshi H, Subbarao S K, Sharma V P
Malaria Research Centre, Delhi, India.
J Am Mosq Control Assoc. 1994 Jun;10(2 Pt 1):225-7.
A 2-site immunoradiometric assay (IRMA) has been done using Anopheles stephensi fed on Plasmodium vivax blood through parafilm to ascertain at what time in the sporogonic cycle circumsporozoite (CS) antigen can be detected, and to detect CS antigen in mosquitoes squashed on filter paper (FP) and cellulose acetate membrane (CAM). The CS antigen was detectable only in mature oocysts, a day prior to sporozoite liberation, and in salivary gland sporozoites. Dissected sporozoites adsorbed on FP/CAM also can be analyzed by IRMA for Plasmodium species identification.
已采用两点免疫放射分析（IRMA），对通过石蜡膜吸食感染间日疟原虫血液的斯氏按蚊进行检测，以确定在孢子生殖周期的何时能够检测到环子孢子（CS）抗原，并检测压在滤纸片（FP）和醋酸纤维素膜（CAM）上的蚊子体内的CS抗原。CS抗原仅在成熟卵囊中（子孢子释放前一天）以及唾液腺子孢子中可检测到。吸附在FP/CAM上的解剖后子孢子也可通过IRMA进行分析，以鉴定疟原虫种类。
