Okazawa H, Yonekura Y, Fujibayashi Y, Yamauchi H, Ishizu K, Nishizawa S, Magata Y, Tamaki N, Fukuyama H, Yokoyama A, Konishi J
Faculty of Medicine, Kyoto University, Japan.
J Nucl Med. 1996 Jul;37(7):1080-5.
We developed copper-62-labeled human serum albumin-dithiosemicarbazone (62Cu-HSA-DTS) as a blood-pool imaging agent for PET. To evaluate 62Cu-HSA-DTS for plasma-pool imaging and to measure the regional cerebral hematocrit, 12 normal volunteers and 7 patients with cerebrovascular disease underwent PET studies with 62Cu-HSA-DTS and 15O-labeled carbon monoxide (C15O).
The normal subjects were studied with both C15O and 62Cu-HSA-DTS. All patients were examined by 15O-gas studies to measure cerebral perfusion and oxygen metabolism, followed by measurement of plasma volume with 62Cu-HSA-DTS for analysis of regional cerebral hematocrit. Regional cerebral hematocrit was calculated from regional cerebral red cell volume (rCRCV) measured by C15O and regional plasma volume (rCPV) measured by 62Cu-HSA-DTS in each subject, and the regional cerebral/large-vessel hematocrit ratio was obtained for both cerebral hemispheres in each subject.
Mean regional cerebral hematocrit and mean cerebral/large-vessel hematocrit ratio in the 12 normal volunteers were 38.3 +/- 3.45% and 0.88 +/- 0.06, respectively. In the seven patients with cerebrovascular disease, regional cerebral hematocrit was significantly lower on the hypoperfused side than the normal hemisphere. The images of rCPV and rCRCV from these patients demonstrated a greater increase in rCPV than rCRCV in the hypoperfused area.
These results suggest that 62Cu-HSA-DTS can be used for measurement of plasma volume and that regional cerebral hematocrit may provide valuable information regarding the microcirculation in the brain.
我们研发了铜 - 62标记的人血清白蛋白 - 二硫代氨基脲（62Cu - HSA - DTS）作为正电子发射断层扫描（PET）的血池显像剂。为评估62Cu - HSA - DTS用于血浆池显像以及测量局部脑血细胞比容，12名正常志愿者和7名脑血管疾病患者接受了使用62Cu - HSA - DTS和15O标记的一氧化碳（C15O）的PET研究。
正常受试者同时接受C15O和62Cu - HSA - DTS研究。所有患者均通过15O气体研究测量脑灌注和氧代谢，随后用62Cu - HSA - DTS测量血浆容量以分析局部脑血细胞比容。根据每个受试者通过C15O测量的局部脑红细胞体积（rCRCV）和通过62Cu - HSA - DTS测量的局部血浆体积（rCPV）计算局部脑血细胞比容，并获得每个受试者两个脑半球的局部脑/大血管血细胞比容比值。
12名正常志愿者的平均局部脑血细胞比容和平均脑/大血管血细胞比容比值分别为38.3±3.45%和0.88±0.06。在7名脑血管疾病患者中，灌注不足侧的局部脑血细胞比容明显低于正常半球。这些患者的rCPV和rCRCV图像显示，灌注不足区域的rCPV增加幅度大于rCRCV。
这些结果表明，62Cu - HSA - DTS可用于测量血浆容量，并且局部脑血细胞比容可能为大脑微循环提供有价值的信息。
