Barquist E, Bonaz B, Martinez V, Rivier J, Zinner M J, Taché Y
Center for Ulcer Research and Education/Digestive Disease Research Center, West Los Angeles Veterans Affairs Medical Center, California, USA.
Am J Physiol. 1996 Apr;270(4 Pt 2):R888-94. doi: 10.1152/ajpregu.1996.270.4.R888.
The 20-min rate of gastric emptying of a noncaloric solution and c-fos expression detected by immunohistochemistry in the brain were monitored 3 h after abdominal surgery performed under 10-min enflurane anesthesia in rats. Abdominal surgery (laparotomy and 1-min manipulation of the cecum) decreased gastric emptying from 60.8 +/- 3.4 to 25.9 +/- 3.4%. Capsaicin applied to the celiac/superior mesenteric ganglia 2 wk before the experiment reduced the delay in gastric emptying induced by abdominal surgery (46.3 +/- 3.4%), whereas perivagal capsaicin application had no effect (23.6 +/- 7.9%). The corticotropin-releasing factor (CRF) antagonist [D-Phe12, Nle21,38,C alpha MeLeu37]CRF-(12--41) injected intracisternally (10-20 micrograms) prevented postoperative gastroparesis induced by surgery, while having no effect on basal gastric emptying. Abdominal surgery increased the number of Fos-positive cells in brain nuclei regulating autonomic outflow: the nucleus of the solitary tract, locus ceruleus, paraventricular nucleus, and supraoptic nucleus of the hypothalamus. These data indicate that capsaicin-sensitive splanchnic afferent fibers and activation of CRF receptors in the brain are part of the neuronal circuitry mediating gastric stasis 3 h after abdominal surgery.
在大鼠接受10分钟安氟醚麻醉后进行腹部手术3小时后，监测了无热量溶液的20分钟胃排空率以及通过免疫组织化学检测的大脑中c-fos表达。腹部手术（剖腹术和1分钟的盲肠操作）使胃排空率从60.8±3.4%降至25.9±3.4%。在实验前2周将辣椒素应用于腹腔/肠系膜上神经节可减轻腹部手术引起的胃排空延迟（46.3±3.4%），而迷走神经周围应用辣椒素则无作用（23.6±7.9%）。脑池内注射促肾上腺皮质激素释放因子（CRF）拮抗剂[D-Phe12，Nle21,38，CαMeLeu37]CRF-(12 - 41)（10 - 20微克）可预防手术引起的术后胃轻瘫，而对基础胃排空无影响。腹部手术增加了调节自主神经输出的脑核中Fos阳性细胞的数量：孤束核、蓝斑、下丘脑室旁核和视上核。这些数据表明，辣椒素敏感的内脏传入纤维和大脑中CRF受体的激活是介导腹部手术后3小时胃潴留的神经回路的一部分。
