Bockmühl U, Schwendel A, Dietel M, Petersen I
Department of Otorhinolaryngology, Humboldt-University Berlin, Germany.
Cancer Res. 1996 Dec 1;56(23):5325-9.
Comparative genomic hybridization was performed on 30 primary head and neck squamous cell carcinomas. Fractional or entire DNA loss of chromosome 3p was a basic finding that occurred in 29 cases (97%). Additional DNA underrepresentations were observed in more than 50% of the cases on chromosomes 1p, 4, 5q, 6q, 8p, 9p, 11q, 13q, 18q, and 21q. Deletions on chromosomes 3p, 13q, and 17p were confirmed by loss of heterozygosity analysis. Entire or partial DNA copy number increases were identified for chromosome 3q in 26 cases (87%) with high-level amplifications at 3q24 and 3q27-qter. Overrepresentations were found in decreasing order of frequency at 11q13 (70%), 8q (57%), 19q (50%), 19p (47%), and 17q (47%). The use of comparative genomic hybridization superkaryograms of the group of well-differentiated carcinomas (G1) indicated that the deletions on chromosomes 3p and 9p along with the overrepresentation of 3q are associated with early tumor development. Accordingly, the undifferentiated tumors (G3) were characterized by additional deletions on chromosomes 4q, 8p, 11q, 13q, 18q, and 21q and overrepresentations on 1pter, 11q13, 19, and 22q, suggesting that these changes are preferentially associated with tumor progression.
对30例原发性头颈部鳞状细胞癌进行了比较基因组杂交分析。3p染色体的部分或全部DNA缺失是一项基本发现，29例（97%）出现该情况。在超过50%的病例中，还观察到1p、4、5q、6q、8p、9p、11q、13q、18q和21q染色体上存在额外的DNA低表达。通过杂合性缺失分析证实了3p、13q和17p染色体上的缺失。在26例（87%）病例中发现3q染色体存在全部或部分DNA拷贝数增加，在3q24和3q27 - qter处有高水平扩增。按频率递减顺序发现11q13（70%）、8q（57%）、19q（50%）、19p（47%）和17q（47%）存在高表达。对高分化癌组（G1）使用比较基因组杂交核型图表明，3p和9p染色体上的缺失以及3q的高表达与肿瘤早期发展相关。因此，未分化肿瘤（G3）的特征是4q、8p、11q、13q、18q和21q染色体上有额外缺失，以及1pter、11q13、19和22q存在高表达，这表明这些变化与肿瘤进展优先相关。
