Kinnaert P, De Wilde J P, Bournonville B, Husson C, Salmon I
Laboratoire Pluridisciplinaire de Recherches Expérimentales Biomédicales, Université Libre de Bruxelles, Belgium.
Ann Surg. 1996 Dec;224(6):749-54; discussion 754-5. doi: 10.1097/00000658-199612000-00010.
The aim of the study was to determine if human peritoneal mesothelial cells (HPMCs) can be activated directly by bacterial products contained in preparations of heat-killed Escherichia coli and staphylococci.
It has been shown recently that cytokine-activated HPMCs produce the inflammatory mediators, interleukin-1, interleukin-6, interleukin-8, and macrophage chemotactic protein-1. Studies concerning the effects of bacterial products on HPMCs are scarce and have not yielded conclusive results.
Growth-arrested HPMC monolayers were prepared from cell suspensions obtained by enzymatic disaggregation of small pieces of omentum. They were incubated for 24 hours with heat-killed E. coli (ATCC 25922), heat-killed staphylococci (ATCC 25933), or E. coli lipopolysaccharide, and the release of various cytokines in the culture media was measured by radioimmunoassays or enzyme-linked immunosorbent assays. Results were expressed as mean +/- standard error of the mean in picograms per milliliter of supernatant and analyzed with the Wilcoxon test; p values of less than 0.05 were considered significant.
Baseline production of interleukin-6, interleukin-8, the chemokine "regulated upon activation, normal T cell expressed and secreted" (RANTES), and macrophage chemotactic protein-1 varied widely from one omental preparation to the other. E. coli increased the release of these mediators: from 1206 +/- 316 pg/mL to 8480 +/- 2189 pg/mL for interleukin-6, from 285 +/- 58 pg/mL to 3164 +/- 1053 pg/mL for interleukin-8, from 7 +/- 5 pg/mL to 684 +/- 264 pg/mL for RANTES, and from 2212 +/- 346 pg/mL to 7726 +/- 1473 pg/mL for macrophage chemotactic protein-1. Heat-killed staphylococci did not alter significantly the production of RANTES or macrophage chemotactic protein-1 but increased the production of the two other cytokines from 1325 +/- 389 pg/mL to 2206 +/- 523 pg/mL for interleukin-6 and from 318 +/- 70 pg/mL to 819 +/- 265 pg/mL for interleukin-8.
The authors' results show that HPMCs are able to react to a direct stimulation with heat-killed microbes. They suggest that HPMCs, as well as resident macrophages, participate actively in the initiation and possibly in the modulation of intraperitonen inflammatory reactions.
本研究旨在确定人腹膜间皮细胞（HPMCs）是否可被热灭活的大肠杆菌和葡萄球菌制剂中所含的细菌产物直接激活。
最近已表明，细胞因子激活的HPMCs可产生炎性介质，白细胞介素-1、白细胞介素-6、白细胞介素-8和巨噬细胞趋化蛋白-1。关于细菌产物对HPMCs影响的研究较少，且未得出确凿结果。
从通过酶解小块大网膜获得的细胞悬液中制备生长停滞的HPMC单层。将它们与热灭活的大肠杆菌（ATCC 25922）、热灭活的葡萄球菌（ATCC 25933）或大肠杆菌脂多糖孵育24小时，通过放射免疫测定法或酶联免疫吸附测定法测量培养基中各种细胞因子的释放。结果以皮克每毫升上清液的平均值±平均标准误差表示，并用Wilcoxon检验进行分析；p值小于0.05被认为具有统计学意义。
白细胞介素-6、白细胞介素-8、趋化因子“活化时正常T细胞表达和分泌的调节因子”（RANTES）和巨噬细胞趋化蛋白-1的基础产量在不同的大网膜制剂之间差异很大。大肠杆菌增加了这些介质的释放：白细胞介素-6从1206±316 pg/mL增加到8480±2189 pg/mL，白细胞介素-8从285±58 pg/mL增加到3164±1053 pg/mL，RANTES从7±5 pg/mL增加到684±264 pg/mL，巨噬细胞趋化蛋白-1从2212±346 pg/mL增加到7726±1473 pg/mL。热灭活的葡萄球菌未显著改变RANTES或巨噬细胞趋化蛋白-1的产生，但使另外两种细胞因子的产生增加，白细胞介素-6从1325±389 pg/mL增加到2206±523 pg/mL，白细胞介素-8从318±70 pg/mL增加到819±265 pg/mL。
作者的结果表明，HPMCs能够对热灭活微生物的直接刺激做出反应。他们认为，HPMCs以及驻留巨噬细胞积极参与腹膜内炎症反应的启动，可能还参与其调节。
