Zhang Y Z, Bjursten L M, Freij-Larsson C, Kober M, Wesslén B
Department of Experimental Research, Malmö General Hospital, Lund University, Sweden.
Biomaterials. 1996 Dec;17(23):2265-72. doi: 10.1016/0142-9612(96)00055-5.
Two different commercial polymeric materials, a silicone and a polyurethane (PUR), were studied with regard to correlations between the chemical and physical compositions of the polymer surfaces and the biological response on implantation. Test specimens of the materials were manufactured according to standard procedures. The specimens were implanted in rats for 10 and 90 days. Before implantation the polymers were sterilized in three different ways, namely, beta irradiation, ethylene oxide sterilization and steam sterilization. The polymers were characterized before and after the implantation with respect to the chemical composition and the morphology of the surfaces. After implantation the biological response was evaluated by counting numbers of macrophages, giant cells, fibroblasts and other cells present at the surfaces. The thickness of the fibrous capsule surrounding the test specimens was measured at the thickest and thinnest parts. PUR surfaces showed signs of degradation already after sterilization and after 10 to 90 days of implantation, pits and cracks appeared, especially in the ethylene oxide sterilized samples. However, differences in the biological responses were small and independent of the sterilization method. After 10 days of implantation the capsule thickness and the amounts of cell material adhering at the surfaces were different, and it appears that the silicone rubber induces more tissue response than PUR. The differences in the early tissue response evened out after 90 days implantation time and a steady state situation evolved, which was similar for the silicone and the polyurethane.
研究了两种不同的商用聚合材料，即硅酮和聚氨酯（PUR），以探讨聚合物表面的化学和物理组成与植入后的生物学反应之间的相关性。材料的测试样本按照标准程序制造。将样本植入大鼠体内10天和90天。植入前，聚合物通过三种不同方式进行灭菌，即β射线辐照、环氧乙烷灭菌和蒸汽灭菌。对聚合物在植入前后的表面化学成分和形态进行了表征。植入后，通过计数表面存在的巨噬细胞、巨细胞、成纤维细胞和其他细胞的数量来评估生物学反应。在测试样本周围纤维囊的最厚和最薄部分测量其厚度。PUR表面在灭菌后以及植入10至90天后就已出现降解迹象，出现了凹坑和裂缝，尤其是在环氧乙烷灭菌的样本中。然而，生物学反应的差异很小，且与灭菌方法无关。植入10天后，囊的厚度和表面附着的细胞物质数量有所不同，似乎硅橡胶比PUR引发更多的组织反应。植入90天后，早期组织反应的差异趋于平稳，出现了一种稳定状态，硅酮和聚氨酯的情况相似。
