Guo W, Willén R, Liu X, Odelius R, Carlén B
Department of Surgery, Lund University, Sweden.
J Biomed Mater Res. 1994 Dec;28(12):1433-8. doi: 10.1002/jbm.820281208.
To study the splenic response to intraperitoneal biomaterials, 100% silicon rubber drain fragments were intraperitoneally implanted in the rat. Four days after implantation, specimens of the spleen and implanted rubber fragment were retrieved and subjected to scanning electron microscopy (SEM) and energy dispersive X-ray microanalysis (EDX). In SEM, macrophages with membrane fusion and cytoplasmic spreading were noted on the surfaces of implanted rubber fragments. Specimens of the spleen from animals with implants showed light 3-10-microns structures that were not observed in those without implants. EDX revealed the presence of silicon both in the rubber fragment and in the spleen of the implanted animals. Both light microscopy and transmission electron microscopy showed a large number of particles inside giant cells of the spleen. The present study demonstrated an active transport of rubber fragments containing silicon from the peritoneal cavity to the spleen by adherent macrophages, reflecting a splenic response to intraperitoneal implantation of biomaterials. The modes of silicon rubber degradation and transportation remain to be elucidated.
为研究脾脏对腹腔内生物材料的反应，将100%硅橡胶引流片植入大鼠腹腔。植入后4天，取出脾脏和植入的橡胶片标本，进行扫描电子显微镜（SEM）和能量色散X射线微分析（EDX）。在扫描电子显微镜下，观察到植入橡胶片表面有膜融合和细胞质伸展的巨噬细胞。植入动物的脾脏标本显示出3至10微米的浅色结构，而未植入动物的脾脏中未观察到这种结构。能量色散X射线微分析显示，植入动物的橡胶片和脾脏中均存在硅。光学显微镜和透射电子显微镜均显示脾脏巨细胞内有大量颗粒。本研究表明，附着的巨噬细胞将含硅的橡胶碎片从腹腔主动转运至脾脏，这反映了脾脏对腹腔内植入生物材料的反应。硅橡胶的降解和运输方式仍有待阐明。
