Straubinger R K, Summers B A, Chang Y F, Appel M J
James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
J Clin Microbiol. 1997 Jan;35(1):111-6. doi: 10.1128/jcm.35.1.111-116.1997.
In specific-pathogen-free dogs experimentally infected with Borrelia burgdorferi by tick exposure, treatment with high doses of amoxicillin or doxycycline for 30 days diminished but failed to eliminate persistent infection. Although joint disease was prevented or cured in five of five amoxicillin- and five of six doxycycline-treated dogs, skin punch biopsies and multiple tissues from necropsy samples remained PCR positive and B. burgdorferi was isolated from one amoxicillin- and two doxycycline-treated dogs following antibiotic treatment. In contrast, B. burgdorferi was isolated from six of six untreated infected control dogs and joint lesions were found in four of these six dogs. Serum antibody levels to B. burgdorferi in all dogs declined after antibiotic treatment. Negative antibody levels were reached in four of six doxycycline- and four of six amoxicillin-treated dogs. However, in dogs that were kept in isolation for 6 months after antibiotic treatment was discontinued, antibody levels began to rise again, presumably in response to proliferation of the surviving pool of spirochetes. Antibody levels in untreated infected control dogs remained high.
在通过蜱虫叮咬实验性感染伯氏疏螺旋体的无特定病原体犬中，用高剂量阿莫西林或强力霉素治疗30天可减少但未能消除持续性感染。尽管在接受阿莫西林治疗的5只犬和接受强力霉素治疗的6只犬中的5只中关节疾病得到了预防或治愈，但皮肤打孔活检以及尸检样本中的多个组织PCR检测仍呈阳性，并且在抗生素治疗后，从1只接受阿莫西林治疗的犬和2只接受强力霉素治疗的犬中分离出了伯氏疏螺旋体。相比之下，在6只未治疗的感染对照犬中，有6只分离出了伯氏疏螺旋体，并且在这6只犬中有4只发现了关节病变。所有犬在抗生素治疗后血清中针对伯氏疏螺旋体的抗体水平均下降。在接受强力霉素治疗的6只犬中有4只以及接受阿莫西林治疗的6只犬中有4只抗体水平降至阴性。然而，在抗生素治疗停止后隔离饲养6个月的犬中，抗体水平再次开始上升，推测这是对存活的螺旋体菌池增殖的反应。未治疗的感染对照犬中的抗体水平仍然很高。
