通过聚合酶链反应（PCR）方法组合对睡眠嗜血杆菌进行基因组指纹分析。
Genomic fingerprinting of Haemophilus somnus by a combination of PCR methods.
作者信息
Appuhamy S, Parton R, Coote J G, Gibbs H A
机构信息
Division of Infection and Immunity, University of Glasgow, United Kingdom.
出版信息
J Clin Microbiol. 1997 Jan;35(1):288-91. doi: 10.1128/jcm.35.1.288-291.1997.
Abstract
Twenty-three isolates of Haemophilus somnus were typed by repetitive extragenic palindromic (REP) element-based PCR, enterobacterial repetitive intergenic consensus (ERIC)-based PCR, and PCR ribotyping. A total of 11 types were distinguished by REP-PCR, 13 types were distinguished by ERIC-PCR, and 5 types were distinguished by PCR ribotyping. PCR ribotyping produced a relatively simple pattern and a small number of distinct types, whereas REP- and ERIC-PCR both produced more complex banding patterns but increased the discrimination between strains. Clearly distinguishable profiles were obtained for respiratory and genital isolates of H. somnus by all three typing methods. The results suggest that a combination of all three primer sets provides a high-resolution fingerprinting method for epidemiological studies of H. somnus and for its differentiation from related species.
摘要
采用基于重复外显子回文序列（REP）的聚合酶链反应（PCR）、基于肠杆菌重复基因间共有序列（ERIC）的PCR和PCR核糖体分型法，对23株睡眠嗜血杆菌分离株进行分型。REP-PCR共区分出11种类型，ERIC-PCR区分出13种类型，PCR核糖体分型区分出5种类型。PCR核糖体分型产生的图谱相对简单，不同类型数量较少，而REP-PCR和ERIC-PCR产生的条带图谱更复杂，但增加了菌株间的鉴别力。通过这三种分型方法，均可获得睡眠嗜血杆菌呼吸道和生殖道分离株清晰可辨的图谱。结果表明，这三种引物组相结合，可为睡眠嗜血杆菌的流行病学研究及其与相关物种的鉴别提供一种高分辨率指纹图谱方法。
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