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用于快速鉴定和表征精液放线杆菌菌株的聚合酶链反应方法。

PCR methods for rapid identification and characterization of Actinobacillus seminis strains.

作者信息

Appuhamy S, Coote J G, Low J C, Parton R

机构信息

Division of Infection and Immunity, University of Glasgow, Scotland, United Kingdom.

出版信息

J Clin Microbiol. 1998 Mar;36(3):814-7. doi: 10.1128/JCM.36.3.814-817.1998.

DOI:10.1128/JCM.36.3.814-817.1998
PMID:9508320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC104633/
Abstract

Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced the simplest pattern and could be useful for identification of A. seminis and for its differentiation from related species. REP- and ERIC-PCR could be used for strain differentiation in epidemiological studies of A. seminis.

摘要

采用PCR核糖体分型、基于重复外显子回文元件（REP）的PCR以及基于肠杆菌重复基因间共有序列（ERIC）的PCR对24株精液放线杆菌进行分型。通过REP-PCR区分出5种类型，通过ERIC-PCR区分出9种类型。PCR核糖体分型产生的图谱最为简单，可用于精液放线杆菌的鉴定及其与相关物种的区分。REP-PCR和ERIC-PCR可用于精液放线杆菌流行病学研究中的菌株鉴别。

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