巴黎一家儿童医院中对青霉素敏感性降低的肺炎链球菌的分子流行病学研究
Molecular epidemiology of Streptococcus pneumoniae with decreased susceptibility to penicillin in a Paris children's hospital.
作者信息
Moissenet D, Valcin M, Marchand V, Garabédian E N, Geslin P, Garbarg-Chenon A, Vu-Thien H
机构信息
Service de Bactériologie-Virologie-Hygiène, Hôpital d'enfants Armand-Trousseau, Paris, France.
出版信息
J Clin Microbiol. 1997 Jan;35(1):298-301. doi: 10.1128/jcm.35.1.298-301.1997.
Abstract
Among pneumococci with decreased susceptibility or pneumococci resistant to penicillin (PRP) isolated at Armand-Trousseau children's hospital, those expressing capsular serotypes 23F, 9V, and 14 were the most frequently isolated. We compared 53 clinical isolates (14 type 9V, 26 type 23F, and 13 type 14) by analysis of chromosomal macrorestriction patterns and DNA restriction patterns of the penicillin-binding protein (PBP) genes pbp 2b, pbp 2x, and pbp 1a. All 9V isolates originated from the same clone. Five 23F clones were distinguished, the largest of which comprised 20 isolates. The main type 14 clone comprised nine isolates; three other type 14 strains were closely related to the 9V clone, probably by horizontal transfer of capsular biosynthesis genes. Most 23F and type 14 isolates shared the same PBP gene restriction patterns as the 9V clone, suggesting horizontal transfer of altered PBP genes.
摘要
在阿曼德-特鲁索儿童医院分离出的对青霉素敏感性降低的肺炎球菌或耐青霉素肺炎球菌（PRP）中，表达23F、9V和14型荚膜血清型的菌株最为常见。我们通过分析染色体宏观限制性图谱以及青霉素结合蛋白（PBP）基因pbp 2b、pbp 2x和pbp 1a的DNA限制性图谱，对53株临床分离株（14株9V型、26株23F型和13株14型）进行了比较。所有9V型分离株均来自同一克隆。区分出了5个23F克隆，其中最大的一个包含20株分离株。主要的14型克隆包含9株分离株；另外3株14型菌株与9V克隆密切相关，可能是通过荚膜生物合成基因的水平转移。大多数23F型和14型分离株与9V克隆具有相同的PBP基因限制性图谱，提示改变的PBP基因发生了水平转移。
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