巴黎一家儿童医院中对青霉素敏感性降低的肺炎链球菌的分子流行病学研究
Molecular epidemiology of Streptococcus pneumoniae with decreased susceptibility to penicillin in a Paris children's hospital.
作者信息
Moissenet D, Valcin M, Marchand V, Garabédian E N, Geslin P, Garbarg-Chenon A, Vu-Thien H
机构信息
Service de Bactériologie-Virologie-Hygiène, Hôpital d'enfants Armand-Trousseau, Paris, France.
出版信息
J Clin Microbiol. 1997 Jan;35(1):298-301. doi: 10.1128/jcm.35.1.298-301.1997.
Abstract
Among pneumococci with decreased susceptibility or pneumococci resistant to penicillin (PRP) isolated at Armand-Trousseau children's hospital, those expressing capsular serotypes 23F, 9V, and 14 were the most frequently isolated. We compared 53 clinical isolates (14 type 9V, 26 type 23F, and 13 type 14) by analysis of chromosomal macrorestriction patterns and DNA restriction patterns of the penicillin-binding protein (PBP) genes pbp 2b, pbp 2x, and pbp 1a. All 9V isolates originated from the same clone. Five 23F clones were distinguished, the largest of which comprised 20 isolates. The main type 14 clone comprised nine isolates; three other type 14 strains were closely related to the 9V clone, probably by horizontal transfer of capsular biosynthesis genes. Most 23F and type 14 isolates shared the same PBP gene restriction patterns as the 9V clone, suggesting horizontal transfer of altered PBP genes.
摘要
在阿曼德-特鲁索儿童医院分离出的对青霉素敏感性降低的肺炎球菌或耐青霉素肺炎球菌（PRP）中，表达23F、9V和14型荚膜血清型的菌株最为常见。我们通过分析染色体宏观限制性图谱以及青霉素结合蛋白（PBP）基因pbp 2b、pbp 2x和pbp 1a的DNA限制性图谱，对53株临床分离株（14株9V型、26株23F型和13株14型）进行了比较。所有9V型分离株均来自同一克隆。区分出了5个23F克隆，其中最大的一个包含20株分离株。主要的14型克隆包含9株分离株；另外3株14型菌株与9V克隆密切相关，可能是通过荚膜生物合成基因的水平转移。大多数23F型和14型分离株与9V克隆具有相同的PBP基因限制性图谱，提示改变的PBP基因发生了水平转移。
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本文引用的文献
1
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J Clin Microbiol. 1993 Oct;31(10):2724-8. doi: 10.1128/jcm.31.10.2724-2728.1993.
2
Genetic diversity of penicillin-binding protein 2B and 2X genes from Streptococcus pneumoniae in South Africa.南非肺炎链球菌青霉素结合蛋白2B和2X基因的遗传多样性
Antimicrob Agents Chemother. 1993 Sep;37(9):1938-44. doi: 10.1128/AAC.37.9.1938.
3
Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.解读脉冲场凝胶电泳产生的染色体DNA限制性图谱：细菌菌株分型标准
J Clin Microbiol. 1995 Sep;33(9):2233-9. doi: 10.1128/jcm.33.9.2233-2239.1995.
4
Horizontal transfer of penicillin-binding protein genes in penicillin-resistant clinical isolates of Streptococcus pneumoniae.肺炎链球菌青霉素耐药临床分离株中青霉素结合蛋白基因的水平转移
Proc Natl Acad Sci U S A. 1989 Nov;86(22):8842-6. doi: 10.1073/pnas.86.22.8842.
5
Extensive re-modelling of the transpeptidase domain of penicillin-binding protein 2B of a penicillin-resistant South African isolate of Streptococcus pneumoniae.一株来自南非的耐青霉素肺炎链球菌分离株的青霉素结合蛋白2B转肽酶结构域的广泛重塑。
Mol Microbiol. 1989 Jan;3(1):95-102. doi: 10.1111/j.1365-2958.1989.tb00108.x.
6
Nucleotide sequences of the pbpX genes encoding the penicillin-binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime-resistant mutant, C506.编码肺炎链球菌R6青霉素结合蛋白2x及头孢噻肟耐药突变体C506的pbpX基因的核苷酸序列。
Mol Microbiol. 1989 Oct;3(10):1337-48. doi: 10.1111/j.1365-2958.1989.tb00115.x.
7
Penicillin-resistant viridans streptococci have obtained altered penicillin-binding protein genes from penicillin-resistant strains of Streptococcus pneumoniae.耐青霉素的草绿色链球菌已从耐青霉素的肺炎链球菌菌株获得了改变的青霉素结合蛋白基因。
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5858-62. doi: 10.1073/pnas.87.15.5858.
8
Intercontinental spread of a multiresistant clone of serotype 23F Streptococcus pneumoniae.23F血清型肺炎链球菌多重耐药克隆株的洲际传播。
J Infect Dis. 1991 Aug;164(2):302-6. doi: 10.1093/infdis/164.2.302.
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Mol Microbiol. 1991 Sep;5(9):2255-60. doi: 10.1111/j.1365-2958.1991.tb02155.x.
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Mol Microbiol. 1991 Aug;5(8):1993-2002. doi: 10.1111/j.1365-2958.1991.tb00821.x.
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