Muñoz R, Coffey T J, Daniels M, Dowson C G, Laible G, Casal J, Hakenbeck R, Jacobs M, Musser J M, Spratt B G
Laboratory of Microbiology, Rockefeller University, New York City, NY 10021.
J Infect Dis. 1991 Aug;164(2):302-6. doi: 10.1093/infdis/164.2.302.
Isolates of serotype 23F Streptococcus pneumoniae with high levels of resistance of penicillin have been commonly recovered in Spain for more than a decade. Recently penicillin-resistant serotype 23F S. pneumoniae strains were also isolated from children attending a day-care center in Cleveland. A number of Spanish and Cleveland isolates were compared by electrophoretic analysis of penicillin-binding protein (PBP) profiles and DNA restriction endonuclease cleavage profiles of the PBP 2X and 2B genes amplified with the polymerase chain reaction and by multilocus enzyme electrophoresis. All strains were identical by these criteria. The findings demonstrate that the Spanish and Cleveland isolates are clonally related and suggest that this antibiotic resistant clone of serotype 23F S. pneumoniae has spread intercontinentally from Spain to the United States.
十多年来，在西班牙普遍分离出对青霉素具有高度耐药性的23F血清型肺炎链球菌菌株。最近，在克利夫兰一家日托中心的儿童中也分离出了对青霉素耐药的23F血清型肺炎链球菌菌株。通过对青霉素结合蛋白（PBP）谱进行电泳分析，以及对用聚合酶链反应扩增的PBP 2X和2B基因的DNA限制性内切酶切割图谱进行分析，并通过多位点酶电泳，对一些西班牙和克利夫兰的分离株进行了比较。根据这些标准，所有菌株都是相同的。这些发现表明，西班牙和克利夫兰的分离株具有克隆相关性，并表明这种23F血清型肺炎链球菌的抗生素耐药克隆已从西班牙跨洲际传播到美国。
